不同粒径零价铁对微氧脱氮系统的影响  

Effects of zero-valent iron with different particle sizes on micro-aerobic denitrification system

作　　者：于雅楠 张潇[1] 岳秀萍[1,2] 赵博玮 周爱娟 崔颖[1] 邢剑波 YU Ya’nan;ZHANG Xiao;YUE Xiuping;ZHAO Bowei;ZHOU Aijuan;CUI Ying;XING Jianbo(School of Environmental Science and Engineering,Taiyuan University of Science and Technology,Jinzhong 030600,China;Shanxi Zhejiang University New Materials and Chemical Research Institute,Taiyuan 030032,China;Datong Ecological Environment Assessment Center,Datong 037002,China)

机构地区：[1]太原理工大学环境科学与工程学院,山西晋中030600 [2]山西浙大新材料与化工研究院,山西太原030032 [3]大同市生态环境评估中心,山西大同037002

出　　处：《工业水处理》2024年第4期84-97,共14页Industrial Water Treatment

基　　金：国家自然科学基金项目(52070139,52270134);山西省基础研究计划项目(20210302124479,202103021224099);山西浙大研究院项目(2022SXTD006);山西省高等学校科技创新项目(jyt202004)。

摘　　要：构建了部分硝化反硝化厌氧氨氧化(SNAD)微氧体系,并定期向体系内投加120 mg不同粒径的零价铁(ZVI),从反应器运行情况、微生物性能以及群落结构来探究不同粒径ZVI对微氧系统的影响。研究结果表明,在50 nm粒径ZVI(50 nZVI)的介导下,系统的TIN去除率最终稳定在83.591%,电子传递活性(ETSA)是接种污泥的1.48倍,均高于其他粒径ZVI的影响。越小粒径ZVI越能促进胞外聚合物的产生,有利于活性污泥结构稳定。这都源于粒径越小的ZVI更容易被微生物吸附、利用。微生物群落结构与功能分析结果表明,在粒径越小的ZVI介导下的微生物群落多样性越高,群落结构也越复杂。ZVI介导会促进对氧需求不敏感的微生物的富集,样品中主要检测出Candidatus_Brocadia和Candidatus_Kuenenia两种厌氧氨氧化菌(AnAOB),且在50 nZVI影响下丰度最高,分别为4.650%和0.692%。nZVI介导下,AnAOB与Gemmobacter和norank_f_Comamonadaceae两种兼性厌氧型反硝化菌共同作用构成SNAD的脱氮途径。而在mZVI介导下,兼性厌氧型反硝化为主要脱氮途径。A simultaneous partial nitrification,anammox and denitrification(SNAD)micro-aerobic system was con-structed.The effects of different particle sizes of ZVI on the micro-aerobic system were investigated from the aspects of operation,microbial performance and community structure by adding 120 mg of zero-valent iron(ZVI)into the sys-tem.The results showed that the TIN removal rate of the system stabilized at 83.591%and the electron transport sys-tem activity(ETSA)was 1.48 times of the inoculated sludge under the domestication of ZVI with a partical size of 50 nm(50 nZVI),which was higher than the influence of other particle sizes of ZVI.The smaller the particle size,the more production of extracellular polymers promoted by ZVI,the more stable of the activated sludge structure.This was due to the fact that ZVI of smaller particle size was more easily adsorbed and utilized by microorganisms.The results of microbial community analysis showed that the smaller the particle size,the higher the diversity of the microbial community mediated by ZVI,and the more complex the community structure.ZVI mediation promoted the enrichment of microorganisms that were not sensitive to oxygen demand.Candidatus_Brocadia and Candidatus_Kue-nenia,two kinds of AnAOB were the main bacteria detected in the samples with the highest abundance of 4.650%and 0.692%under the influence of 50 nZVI.Under nZVI,AnAOB acted together with two facultatively anaerobic denitrifying bacteria,Gemmobacter and norank_f_Comamonadaceae,which constituted the denitrification pathway of SNAD.In contrast,facultatively anaerobic denitrification was the main denitrification pathway mediated by mZVI.

关 键 词：微氧 部分硝化反硝化厌氧氨氧化系统 零价铁 电子传递活性 生物脱氮 

分 类 号：X703[环境科学与工程—环境工程]