Noria-PEI改性PVA载体固定化溶菌酶用于污泥发酵产VFAs研究  

Study on VFAs production of sludge fermentation with the immobiliza⁃tion of lysozyme on Noria-PEI modified PVA carrier

作　　者：王昊龙 张恩泽 王梓诚 赵瑜涵 南军 孙彦民 马爱静 周立山 刘丽强 蔡巷 段兴宇 WANG Haolong;ZHANG Enze;WANG Zicheng;ZHAO Yuhan;NAN Jun;SUN Yanmin;MA Aijing;ZHOU Lishan;LIU Liqiang;CAI Xiang;DUAN Xingyu(CenerTech Tianjin Chemical Research and Design Institute Co.,Ltd.,Tianjin 300131,China;State Key Laboratory of Separation Membranes and Membrane Processes,School of Chemical Engineering and Technology,Tiangong University,Tianjin 300387,China;School of Chemical Engineering,Hebei University of Technology,Tianjin 300401,China)

机构地区：[1]中海油天津化工研究设计院有限公司,天津300131 [2]天津工业大学化学工程与技术学院,分离膜与膜过程国家重点实验室,天津300387 [3]河北工业大学化工学院,天津300401

出　　处：《工业水处理》2024年第4期155-163,共9页Industrial Water Treatment

基　　金：海油发展基础前瞻项目(HFKJ-CG-TJY-2022-01)。

摘　　要：生物酶促进污泥厌氧消化具有反应温和、高效环保等优点,然而回收困难和催化稳定性差严重制约其应用,固定化酶是解决上述问题的关键。采用水轮酚(Noria)和聚乙烯亚胺(PEI)共沉积法改性聚乙烯醇(PVA)载体,并研究了Noria-PEI改性PVA载体固定溶菌酶对污泥厌氧发酵产挥发性脂肪酸(VFAs)效能的影响。结果表明,固定化溶菌酶可强化污泥发酵提高SCOD,在36 h内SCOD最高浓度达到2 892.4 mg/L,相比空白组提升3.4倍(第6天),VFAs质量浓度在第4天达到峰值2 130.8 mg/L,乙酸和丙酸占比达到61.8%~80.7%。固定酶系统中污泥的平均粒径为42.957μm,比表面积为0.413 m^(2)/g,固定化溶菌酶强化了颗粒态污泥水解。微生物群落结构分析显示,在固定酶系统中Firmicutes、Proteobacteria和Bacteroides等水解产酸菌门占比较高;在属水平上,以Macellibacteroides、Petrimonas、Lactobacillus和Clostridium_sensu_stricto_1菌属为主,从微观层面上解释了固定酶强化污泥发酵产酸机理。Sludge anaerobic digestion promoted by enzymes has the advantages of mild reaction,high efficiency and environmental protection etc.However,the difficulty of recovery and poor catalytic stability seriously restrict its ap-plication.Enzyme immobilization is the key to solve the above problems.A new type of polyvinyl alcohol(PVA)car-rier was prepared by Noria and polyethylenimide(PEI)co-deposition.The effect of lysozyme immobilized with Noria-PEI modified PVA carriers on the efficiency of sludge anaerobic fermentation for VFAs production was studied.The results showed that immobilized lysozyme could enhance sludge fermentation to promote SCOD,and SCOD reached the highest concentration of 2892.4 mg/L within 36 h,which was 3.4 times higher than in the blank(day 6).VFAs concentration peaked at 2130.8 mg/L on day 4,mainly comprised of acetic acid and propionic acid,accounting for 61.8%-80.7%.The average particle size and specific surface area of sludge in the immobilized enzyme system were 42.957µm and 0.413 m^(2)/g,indicating that immobilized enzyme enhanced the hydrolysis of granular sludge.Micro-bial community analysis showed that the hydrolytic acid-producing phyla of Firmicutes,Proteobacteria and Bacteroi-des accounted for high proportion in the immobilized enzyme system.At the genus level,Macellibacteroides,Petri-mona,Lactobacillu,and Clostridium_sensu_stricto_1 were the main genera,which revealed the mechanism of en-hanced VFAs production by the immobilized enzyme at microscopic level.

关 键 词：水轮酚 聚乙烯醇载体 固定酶 剩余污泥 挥发性脂肪酸 

分 类 号：X703[环境科学与工程—环境工程]