靶向编辑O⁃GlcNAc糖基化修饰的化学生物学技术  

Chemical Biology Techniques for Targeted Manipulation of O-GlcNAcylation

作　　者：潘雅文 王贞超[1] 沈大成 PAN Ya-Wen;WANG Zhen-Chao;SHEN Da-Cheng(School of Pharmaceutical Sciences,Guizhou University,Guiyang 550025,China;Institute of Chemical Biology,Shenzhen Bay Laboratory,Shenzhen 518132,China)

机构地区：[1]贵州大学药学院,贵阳550025 [2]深圳湾实验室化学生物学研究所,深圳518132

出　　处：《中国生物化学与分子生物学报》2024年第4期453-462,共10页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金青年科学基金(No.22007022,No.22307082)资助。

摘　　要：糖基化修饰是最丰富和最复杂的蛋白质翻译后修饰之一。其中,氧连-N-乙酰氨基葡萄糖基化修饰(O-GlcNAcylation)作为广泛存在于真核细胞质或线粒体的蛋白质糖修饰,影响了蛋白质性质、细胞功能和疾病状态等方面,因此,在活细胞中的靶蛋白质上进行编辑O-GlcNAc糖基化对于与其相关的功能调控至关重要。这些在细胞靶蛋白质上操控O-GlcNAc糖基化修饰的新技术,将在很大程度上加速O-GlcNAc功能研究的发展。本文简要介绍了近年来靶向编辑O-GlcNAc糖基化修饰化学生物学技术的研究进展,讨论了目前可用的O-GlcNAc编辑策略并进行了分析,展望了相关技术的未来发展前景。这些技术组成了一个强大的化学生物学工具箱,并有望用于与O-GlcNAc糖基化相关疾病的诊断与治疗。O-linked-N-acetylglucosaminylation(O-GlcNAcylation)is a type of glycosylation that widely present in eukaryotic cytoplasmic proteins or mitochondria.It affects protein properties,cell functions,disease states and so on,hence it is import to identify the roles that O-GlcNAcylations play on target proteins in living cells.The new technologies for manipulating O-GlcNAcylations on target proteins will greatly accelerate the understanding of O-GlcNAc’s functions.This article briefly introduces some recent research progress of chemical biology techniques for targeted protein O-GlcNAcylation,and also analyzes the advantages and limitations of these strategies,and their future development prospects.These technologies build a powerful chemical biology toolbox,which may contribute to the diagnoses and treatments related to O-GlcNAcylation.

关 键 词：O-GLCNAC 糖基化修饰 靶向编辑 化学生物学 

分 类 号：Q503[生物学—生物化学]