电针对三叉神经痛大鼠三叉神经节P2X4R-p38 MAPK-BDNF信号通路的影响  

Effect of electroacupuncture on P2X4R-p38 MAPK-BDNF signaling pathway in trigeminal ganglion of rats with trigeminal neuralgia

作　　者：王祥[1] 霍建忠[2] 韩冲芳[3] 杨文曲[3] 贺建东[3] Wang Xiang;Huo Jianzhong;Han Chongfang;Yang Wenqu;He Jiandong(Department of Pain Medicine,Third Hospital of Shanxi Medical University,Shanxi Bethune Hospital,Taiyuan 030032,China;Department of Orthopedics,Shanxi Medical University Affiliated Taiyuan Central Hospital,Taiyuan Central Hospital,Taiyuan 030006,China;Department of Anesthesiology,Third Hospital of Shanxi Medical University,Shanxi Bethune Hospital,Taiyuan 030032,China)

机构地区：[1]山西医科大学第三医院山西白求恩医院疼痛科,太原030032 [2]山西医科大学附属太原中心医院太原市中心医院骨科,太原030006 [3]山西医科大学第三医院山西白求恩医院麻醉科,太原030032

出　　处：《中华麻醉学杂志》2024年第3期324-328,共5页Chinese Journal of Anesthesiology

基　　金：山西省基础研究计划项目(202103021223410)。

摘　　要：目的评价电针对三叉神经痛大鼠三叉神经节离子型嘌呤受体4(P2X4R)-p38丝裂原活化蛋白激酶(p38 MAPK)-脑源性神经营养因子(BDNF)信号通路的影响。方法清洁级健康成年雄性SD大鼠36只,体质量190~230 g,2~3月龄,采用随机数字表法分为3组(n=12):假手术组(S组)、三叉神经痛组(TN组)和电针组(E组)。采用三叉神经眶下支慢性缩窄术制备三叉神经痛模型。E组于造模后行患侧百会穴、下关穴电针刺激20 min,频率80 Hz,2次/d,连续14 d。于造模前1 d、造模后3、7、14、21和28 d时测定面部机械痛阈(FMT)。末次行为学测定结束后,处死大鼠取三叉神经节,采用Western blot法检测P2X4R、p38 MAPK、磷酸化p38 MAPK(p-p38 MAPK)和BDNF表达,采用ELISA法检测TNF-α、IL-1β和IL-6含量,HE染色观察三叉神经节组织病理变化。结果与S组比较,TN组造模后各时点FMT降低,三叉神经节P2X4R、p-p38 MAPK和BDNF表达上调,TNF-α、IL-1β和IL-6含量升高(P<0.05),三叉神经节病理学损伤明显;与TN组比较,E组造模后各时点FMT升高,三叉神经节P2X4R、p-p38 MAPK和BDNF表达下调,TNF-α、IL-1β和IL-6含量降低(P<0.05),三叉神经节病理学损伤减轻。结论电针减轻大鼠三叉神经痛的机制可能与抑制三叉神经P2X4R-p38 MAPK-BDNF信号通路活性,减轻神经炎症有关。Objective To evaluate the effect of electroacupuncture on P2X4R-p38 mitogen-activated protein kinase(p38 MAPK)-brain-derived neurotrophic factor(BDNF)signaling pathway in trigeminal ganglion of rats with trigeminal neuralgia.Methods Thirty-six clean-grade healthy adult male Sprague-Dawley rats,weighing 190-230 g,aged 2-3 months,were divided into 3 groups(n=12 each)using a random number table method:sham operation group(S group),trigeminal neuralgia group(TN group),and electroacupuncture group(E group).The model was developed by chronic constriction of the infraorbital nerve in anesthetized animals.The infraorbital nerve was only exposed without ligation in group S.Rats received electroacupuncture stimulation at the Baihui and Xiaguan acupoints on the affected side for 20 min after developing the model,with a frequency of 80 Hz,twice a day,for 14 consecutive days in E group.Facial mechanical pain threshold(FMT)was measured at 1 day before developing the model and 3,7,14,21 and 28 days after developing the model.The rats were sacrificed after the last behavioral testing,and the trigeminal ganglia were taken for examination of histopathological changes of trigeminal ganglion(by HE staining)and for determination of the expression of P2X4R,p38 MAPK,phosphorylated p38 MAPK(p-p38 MAPK)and BDNF(by Western blot)and contents of tumor necrosis factor-alpha(TNF-α),interleukin-1beta(IL-1β)and IL-6(by enzyme-linked immunosorbent assay).Results Compared with group S,the FMT was significantly decreased at each time point after developing the model,the expression of P2X4R,p-p38 MAPK and BDNF in trigeminal ganglion was up-regulated,and the contents of TNF-α,IL-1βand IL-6 were increased(P<0.05),the pathological changes of the trigeminal ganglion were obvious in group TN.Compared with group TN,the FMT was significantly increased at each time point after developing the model,and the expression of P2X4R,p-p38 MAPK and BDNF in trigeminal ganglion was down-regulated,and the contents of TNF-α,IL-1βand IL-6 were decreased(P<0.05),an

关 键 词：电刺激疗法 三叉神经痛 受体 嘌呤能P2X4 P38丝裂原活化蛋白激酶 脑源性神经营养因子 

分 类 号：R245[医药卫生—针灸推拿学]