还原菌Staphylococcus sp.修复六价铬污染土壤的中试研究  

Pilot test of remediation of hexavalent chromium from soil by reducing bacteria strain Staphylococcus sp

作　　者：宋骏杰 许奕敏 李伟平 谢荣焕 刘桂建[2,3] SONG Junjie;XU Yimin;LI Weiping;XIE Ronghuan;LIU Guijian(Anhui Guozhen Environmental Remediation Co.,Ltd.,Hefei 230088,China;School of Earth and Space Sciences,University of Science and Technology of China,Hefei 230026,China;Hefei Engineering Research Center for Soil and Groundwater Remediation,Hefei 230088,China)

机构地区：[1]安徽国祯环境修复股份有限公司,合肥230088 [2]中国科学技术大学地球和空间科学学院,合肥230026 [3]合肥市土壤及地下水修复工程技术研究中心,合肥230088

出　　处：《安全与环境学报》2024年第4期1581-1586,共6页Journal of Safety and Environment

基　　金：合肥市关键共性技术研发项目(2021GJ063);安徽省科技重大专项(202003a06020024)。

摘　　要：为探索微生物修复的工程化应用前景,开展六价铬污染土壤的微生物还原修复中试研究。通过采集废弃电镀厂场地污染土壤,筛选出1株具有六价铬还原作用的功能菌Staphylococcus sp.。Staphylococcus sp.对六价铬的耐受质量浓度高于200 mg/L,最适pH值为7,适宜温度为25~35℃。研究发现,当土壤六价铬初始质量比为98.00 mg/kg、水土比(mL∶g)为2∶1、添加肉膏蛋白胨(Luria-Bertani,LB)培养基的条件下,Staphylococcus sp.能在21 d内将六价铬全部还原。在污染土壤的微生物治理中试研究中,污染土壤的用量为200 kg,水土比(mL∶g)为2∶1,且周期性地添加LB培养基。土壤中六价铬初始质量比为65.42 mg/kg,Staphylococcus sp.在6 d内对六价铬的还原率达到92.59%,后续24 d土壤中六价铬质量比在2.97~10.85 mg/kg呈现波动状态。结果表明,污染场地筛选出的土著功能菌Staphylococcus sp.具有潜在的工程应用价值。中试研究确定的相关参数能为后期工程化应用提供技术支撑。To investigate the application potential of microbial remediation in engineering,a pilot test of remediation of hexavalent chromium from the soil by reducing bacteria was studied.The polluted soil sample was collected from a bankrupt electroplate factory.After consecutive cultivation,a bacteria strain that could reduce hexavalent chromium was isolated.This strain was identified as Staphylococcus sp.through 16S rRNA sequencing.The influence of different factors on the remediation efficiency of Staphylococcus sp.was analyzed,including initial pollutant mass concentration,pH,and temperature.Staphylococcus sp.could survive in 200 mg/L hexavalent chromium solution during the experiment.When pH was 7 and temperature ranged from 25℃to 35℃,the reduction rate of hexavalent chromium was highest.While the initial pollutant mass concentration in water was lower than 100 mg/L,Staphylococcus sp.could reduce all of the hexavalent chromium for 12 days.Under the condition that the initial pollutant mass ratio was 98.00 mg/kg,the water-soil ratio was 2:1,and LB medium was added.Staphylococcus sp.could reduce all of the hexavalent chromium in the soil for 21 days.In the pilot test,200 kg of polluted soil was used to evaluate the remediation efficiency.The water-soil ratio was also adjusted to 2:1,and the LB medium was added periodically.The initial mass ratio of hexavalent chromium in soil was 65.42 mg/kg,and Staphylococcus sp.reduced 92.59%of hexavalent chromium in 6 days.After that,the mass ratio fluctuated between 2.97 mg/kg and 10.85 mg/kg.These might be influenced by the heterogeneous distribution of hexavalent chromium in soil with large amounts.Besides,other bacteria strains in the soil could also impact the remediation efficiency of Staphylococcus sp.The results indicate that the functional bacteria strain Staphylococcus sp.isolated from the polluted site has potential application in remediation engineering.The relevant parameters used in the pilot test can provide technical support in future engineering.

关 键 词：环境工程学 还原菌 六价铬 土壤修复 中试研究 

分 类 号：X53[环境科学与工程—环境工程]