UPLC-MS/MS法同时测定百合知母汤中10种成分的含量  

Simultaneous content determination of ten constituents in Baihe Zhimu Decoction by UPLC-MS/MS

作　　者：喻明洁 来小丹 刘职瑞 范群红 程林 枉前 YU Ming-jie;LAI Xiao-dan;LIU Zhi-rui;FAN Qun-hong;CHENG Lin;WANG Qian(Department of Pharmacy,The First Affiliated Hospital of Army Medical University,Chongqing 400038,China;Department of Pharmacy,NO.958 Hospital of PLA Army,Chongqing 400020,China)

机构地区：[1]陆军军医大学第一附属医院药学部,重庆400038 [2]陆军第九五八医院药学部,重庆400020

出　　处：《中成药》2024年第4期1073-1077,共5页Chinese Traditional Patent Medicine

基　　金：重庆市卫生健康委中医科研项目(2023WSJK128);重庆市临床药学重点专科项目(综合项目)(425ZM2);重庆市科卫联合中医药科研项目(2019ZY3483)。

摘　　要：目的 建立UPLC-MS/MS法同时测定百合知母汤中菝契皂苷元、王百合苷C、知母皂苷AⅢ、芒果苷、新芒果苷、王百合苷A、王百合苷B、知母皂苷BⅢ、异芒果苷、知母皂苷BⅡ的含量。方法 分析采用Agilent Poroshell 120 EC-C_(18)色谱柱(3.0 mm×150 mm, 2.7μm);流动相水(含0.1%甲酸)-乙腈(含0.1%甲酸),梯度洗脱;体积流量0.5 mL/min;柱温30℃;电喷雾离子源;正负离子扫描;多反应监测模式。结果 10种成分在各自范围内线性关系良好(r>0.998 1),平均加样回收率95.65%~106.5%,RSD 1.2%~5.5%。结论 该方法准确、灵敏、快速,可用于百合知母汤的质量控制。AIM To establish a UPLC-MS/MS method for the simultaneous content determination of sarsasapogenin,regaloside C,timosaponin AⅢ,mangiferin,neomangiferin,regaloside A,regaloside B,timosaponin BⅢ,isomangiferin and timosaponin BⅡin Baihe Zhimu Decoction.METHODS The analysis was performed on a 30℃thermostatic Agilent Poroshell 120 EC-C_(18) column(3.0 mm×150 mm,2.7μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile(containing 0.1%formic acid)flowing at 0.5 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Ten constituents showed good linear relationships within their own ranges(r>0.9981),whose average recoveries were 95.65%-106.5%with the RSDs of 1.2%-5.5%.CONCLUSION This accurate,sensitive and rapid method can be used for the quality control of Baihe Zhimu Decoction.

关 键 词：百合知母汤 化学成分 含量测定 UPLC-MS/MS 

分 类 号：R927.2[医药卫生—药学]