一测多评法同时测定心脑康胶囊中9种成分  

Simultaneous determination of nine constituents in Xinnaokang Capsules by QAMS

作　　者：刘康康 靳婉君 浩文婷 刘富强 赖晶 蔺瑞丽 倪琳 LIU Kang-kang;JIN Wan-jun;HAO Wen-ting;LIU Fu-qiang;LAI Jing;LIN Rui-li;NI Lin(College of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;State Key Laboratory for Quality Control of Chinese Medicinal Materials and Decoction Pieces,Gansu Provincial Engineering Laboratory for Chinese and Tibetan Medicine Inspection and Testing Technology,Gansu Provincial Institute for Drug Control,Lanzhou 730030,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省药品检验研究院,国家中药材及饮片质量控制重点实验室,甘肃省中藏药检验检测技术工程实验室,甘肃兰州730030

出　　处：《中成药》2024年第4期1077-1083,共7页Chinese Traditional Patent Medicine

基　　金：甘肃省药品科研项目(2022GSMPA-KL12,2022GSMPA-KL14)。

摘　　要：目的 建立一测多评法同时测定心脑康胶囊中丹参素钠、丹酚酸B、芍药苷、阿魏酸、羟基红花黄色素A、2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、斯皮诺素、甘草苷、葛根素的含量。方法 分析采用SVEA C_(18)色谱柱(250 mm×4.6 mm, 5μm);流动相乙腈-0.2%甲酸,梯度洗脱;体积流量0.5 mL/min;柱温30℃;检测波长280、403 nm。以葛根素为内标,计算其他8种成分的相对校正因子,计算其含量。结果 9种成分在各自范围内线性关系良好(r≥0.999 4),平均加样回收率99.83%~101.18%,RSD 1.04%~1.91%。一测多评法所得结果与外标法接近。结论 该方法准确稳定,重复性好,可用于心脑康胶囊的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of sodium danshensu,salvianolic acid B,paeoniflorin,ferulic acid,hydroxysafflor yellow A,2,3,5,4′-tetrahydroxy stilbene-2-Ο-β-D-glucoside,spinosin,liquiritin and puerarin in Xinnaokang Capsules.METHODS The analysis was performed on a 30℃thermostatic SVEA C_(18) column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.2%formic acid flowing at 0.5 mL/min in a gradient elution manner,and the detection wavelengths were set at 280,403 nm.Puerarin was used as an internal standard to calculate the relative correction factors of the other eight constituents,after which the content determination was made.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.9994),whose average recoveries were 99.83%-101.18%with the RSDs of 1.04%-1.91%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Xinnaokang Capsules.

关 键 词：心脑康胶囊 化学成分 含量测定 一测多评 

分 类 号：R927.2[医药卫生—药学]