UPLC-Q-TOF-MS/MS法分析紫辛鼻鼽颗粒化学成分及入血成分  

Analyses of chemical constituents and constituents absorbed into blood in Zixin Biqiu Granules

作　　者：康雨 胡军华[2] 章晨峰[2] 肖伟[1,2] 戴翠红 林夏[2] 王振中[1,2] KANG Yu;HU Jun-hua;ZHANG Chen-feng;XIAO Wei;DAI Cui-hong;LIN Xia;WANG Zhen-zhong(Nanjing University of Chinese Medicine,Nanjing 210000,China;National Key Laboratory for Technologies of Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacturing,Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China)

机构地区：[1]南京中医药大学,江苏南京210000 [2]江苏康缘药业股份有限公司,中药制药过程控制与智能制造技术全国重点实验室,江苏连云港222001

出　　处：《中成药》2024年第4期1093-1102,共10页Chinese Traditional Patent Medicine

基　　金：国家中医药管理局岐黄学者项目(2021)。

摘　　要：目的 建立UPLC-Q-TOF-MS/MS法分析紫辛鼻鼽颗粒化学成分及入血成分。方法 分析采用Agilent ZORBAX SB-C_(18)色谱柱(1.8μm, 2.1 mm×100 mm);流动相0.2%甲酸-乙腈,梯度洗脱;体积流量0.2 mL/min;柱温30℃;电喷雾离子源;正负离子扫描。根据准分子离子、保留时间、二级碎片离子结合对照品信息及文献报道进行成分鉴定。结果 共鉴定出88种成分,其中28种经对照品比对确认。从大鼠血清中发现了30种成分,其中20种为原型成分,10种为代谢产物。结论 该方法稳定可靠,可为紫辛鼻鼽颗粒药效物质基础、质量控制研究奠定基础。AIM To establish a UPLC-Q-TOF-MS/MS method for the analyses of chemical constituents and constituents absorbed into blood in Zixin Biqiu Granules.METHODS The analysis was performed on a 30℃thermostatic Agilent ZORBAX SB-C_(18) column(1.8μm,2.1 mm×100 mm),with the mobile phase comprising of 0.2%formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode,after which constituent identification was employed according to excimer ions,retention time,secondary fragment ions combined with reference substance information,relevant literatures.RESULTS Total 88 constituents were identified,28 of which were validated by comparison of reference substances.Thirty constituents were observable in rat serum,20 of which were prototype constituents,10 of which were metabolites.CONCLUSION This stable and reliable method can lay the foundation for research on pharmacological substance basis and quality control of Zixin Biqiu Granules.

关 键 词：紫辛鼻鼽颗粒 化学成分 入血成分 UPLC-Q-TOF-MS/MS 

分 类 号：R284.1[医药卫生—中药学]