孕康口服液对缺铁性贫血孕鼠产后恢复及子代质量的影响  

Effects of Yunkang Oral Liquid on postpartum recovery and offspring quality of pregnant mice with iron deficiency anemia

作　　者：李婷 颜美秋 苏洁[1] 郭静妍 汪晨兴 韩丽萍 俞静静 陈素红 吕圭源[1] 楼招欢 LI Ting;YAN Mei-qiu;SU Jie;GUO Jing-yan;WANG Chen-xing;HAN Li-ping;YU Jing-jing;CHEN Su-hong;L Gui-yuan;LOU Zhao-huan(College of Pharmacy,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州310053

出　　处：《中成药》2024年第4期1151-1161,共11页Chinese Traditional Patent Medicine

基　　金：浙江省省级重点实验室项目(2012E10002)。

摘　　要：目的 探究孕康口服液对缺铁性贫血(IDA)孕鼠产后恢复及子代质量的影响。方法 采用饲喂缺铁饲料联合放血法建立缺铁性贫血孕鼠模型,造模成功后,在妊娠第7天同期灌胃给予琥珀酸亚铁片(52 mg/kg)及孕康口服液(9、6、4 mL/kg),每天1次,直至产后6周。结束后,检测小鼠相关体征指标(自主活动、抓力、背温、爪色)、脏器系数(心脏、脾、胸腺系数)、外周血指标(RBC、HGB、HCT、MCH、MCHC)、铁代谢相关血清因子指标(EPO、FEP、SF、sTfR、TFR1),免疫组化法检测肝组织FPN、Tf蛋白表达,免疫荧光法检测肝组织TFR1、Hepcidin蛋白表达,RT-qPCR检测肝组织Hepcidin、FPN、Tf、TFR1 mRNA表达,测定子代相关情况(存活率、自主活动、抓力、爪色、心脏系数、脾脏系数、体长、外周血象指标)。结果 孕康口服液可提高IDA孕鼠产后的自主活动次数、抓力、背温、爪色(P<0.05,P<0.01),降低心脏和脾脏系数(P<0.01),提高全血RBC、HGB、HCT、MCH、MCHC水平(P<0.05,P<0.01),降低血清EPO、FEP、TFR1、sTfR水平(P<0.05,P<0.01),升高SF水平(P<0.05,P<0.01),提高肝组织Hepcidin、FPN mRNA及蛋白表达(P<0.05,P<0.01),降低Tf、TFR1 mRNA及蛋白表达(P<0.05,P<0.01)。与模型组比较,孕康口服液各剂量组子代存活率、自主活动次数、抓力提高(P<0.05,P<0.01),心脏系数、脾脏系数降低(P<0.05,P<0.01),体长增加(P<0.05,P<0.01),全血RBC、HGB、HCT水平升高(P<0.01)。结论 孕康口服液可改善IDA孕鼠的贫血状况,促进其产后恢复,并提高子代质量。AIM To explore the effects of Yunkang Oral Liquid on postpartum recovery and offspring quality of pregnant mice with iron deficiency anemia(IDA).METHODS A pregnant mouse model of IDA was established by feeding of iron deficiency food combined with bloodletting.After successful modeling,ferrous succinate tablets(52 mg/kg)or Yunkang Oral Liquid(9,6,4 mL/kg)was given by gavage on the 7th day of pregnancy,once daily until 6 weeks after delivery followed by the examination of the related signs of mice(spontaneous behaviors,grasping force,back temperature,claw color);and the measurment of levels of organ indices(heart,spleen,thymus coefficient),peripheral blood indices(RBC,HGB,HCT,MCH,MCHC),and serum factors related to iron metabolism(EPO,FEP,SF,sTfR,TFR1).The mice also had their hepatic expressions of FPN and Tf proteins detected by immunohistochemistry;their hepatic protein expressions of TFR1 and Hepcidin detected by immunofluorescence;their hepatic mRNA expressions of Hepcidin,FPN,Tf and TFR1 determined by RT-qPCR;and their offspring related conditions(survival rate,spontaneous behaviors,grip strength,claw color,heart coefficient,spleen coefficient,body length,peripheral blood indices levels)measured as well.RESULTS Yunkang Oral Liquid increased the number of spontaneous activities,grasping force,back temperature and claw color of pregnant rats with IDA after delivery(P<0.05,P<0.01);reduced their heart and spleen coefficient(P<0.01);increased their levels of RBC,HGB,HCT,MCH and MCHC in whole blood(P<0.05,P<0.01);reduced their serum levels of EPO,FEP,TFR1 and sTfR(P<0.05,P<0.01),and increased their SF level(P<0.05,P<0.01);increased their hepatic mRNA expressions of Hepcidin and FPN(P<0.05,P<0.01),and reduced their mRNA and protein expressions of Tf and TFR1(P<0.05,P<0.01).In addition,compared with the offsprings of the model group,those of each Yunkang Oral Liquid group shared improved survival rate;increased number of spontaneous behaviors and grip strength(P<0.05,P<0.01);decreased levels of heart coefficient and s

关 键 词：孕康口服液 缺铁性贫血 铁调素-膜铁转运铁蛋白轴 子代质量 

分 类 号：R285.5[医药卫生—中药学]