长叶轮钟草根腐病病原菌分离鉴定及生物学特性研究  

Study on Isolation,Identification and Biological Characteristics of Root Rot Pathogen from Campanumoea lancifolia

作　　者：廖洪新 凌翠琼 闻进蕊 聂红艳 徐福荣 吕小满 董鲜 LIAO Hong-xin;LING Cui-qiong;WEN Jin-rui;NIE Hong-yan;XU Fu-rong;LYU Xiao-man;DONG Xian(School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500

出　　处：《中药材》2023年第9期2117-2122,共6页Journal of Chinese Medicinal Materials

基　　金：2021云南省重大专项(生物医药)(202102AA310045-02);云南省王源超专家工作站项目(202305AF150018);国家自然科学基金项目(82060683)。

摘　　要：目的:分离、鉴定长叶轮钟草根腐病病原,并研究其生物学特性。方法:采用PDA培养基,从感染了根腐病的长叶轮钟草根系进行病原菌分离纯化及致病力测定,同时进行形态和分子生物学鉴定,并测定两株病原菌的生长曲线、产孢量和镰刀菌酸产量。结果:从发病的长叶轮钟草根系分离鉴定到两株病原菌,将两株菌回接后都能引发长叶轮钟草根系病变,发生褐变干枯,菌株ClF02的致病力强于ClF01。经形态学和分子生物学鉴定,菌株ClF01、ClF02分别为尖孢镰刀菌Fusarium oxysporum和芬芳镰刀菌Fusarium redolens。两株菌的外观、菌丝、孢子等形态特征存在差异,易区分。在相同培养条件下,尖孢镰刀菌和芬芳镰刀菌的生长速率和孢子产量无显著差异,而尖孢镰刀菌镰刀菌酸的产量为芬芳镰刀菌的2.55倍。结论:分离到的两株病原菌分别为尖孢镰刀菌和芬芳镰刀菌,均为导致长叶轮钟草根腐病的病原菌,并了解了这两株菌的生物学特性,为长叶轮钟草根腐病的防治奠定了理论基础。Objective:To isolate and identify the pathogenic pathogen of root rot disease from Campanumoea lancifolia,to study its biological characteristics.Methods:The PDA medium was used to isolate and purify pathogenic pathogen in the root system of the Campanumoea lancifolia with root rot,and the morphological and molecular biology identification and pathogenicity determination were carried out.The growth curve,spore output,fusaric acid yield of the two strains were determined.Results:Two pathogenic fungi were isolated and identified from the diseased root system of Campanumoea lancifolia.Both strains were able to cause root lesions,brown and dry,and the pathogenicity of ClF02 was stronger than ClF01.The strains ClF01 and ClF02 were identified by morphology and molecular biology as Fusarium oxysporum and Fusarium redolens,respectively.The appearance,mycelia,spores and other morphological characteristics of the two kinds of bacteria were different and easy to distinguish.Under the same culture conditions,there was no significant difference in the growth rate and spore output between Fusarium oxysporum and Fusarium redolens,while fusaric acid yield of Fusarium oxysporum was 2.55 times than of Fusarium redolens.Conclusion:The two pathogens isolated in this study are Fusarium oxysporum and Fusarium redolens,respectively,both of which are pathogenic to the root rot of Campanumoea lancifolia.The biological characteristics of these two strains are understood,which lay a theoretical foundation for prevention and control of Campanumoea lancifolia root rot.

关 键 词：长叶轮钟草 根腐病 尖孢镰刀菌 芬芳镰刀菌 镰刀菌酸 

分 类 号：S432.4[农业科学—植物病理学]