基于ICP-MS的不同产地丹参元素差异分析及健康风险评估  

Element Difference Analysis and Health Risk Assessment of Salviae Miltiorrhizae Radix et Rhizoma from Different Producing Areas Based on ICP-MS

作　　者：王巧 于永杰 王紫怡 杨程远 周利[2] 郝庆秀[2] 杨健[2] 黄璐琦 WANG Qiao;YU Yong-jie;WANG Zi-yi;YANG Cheng-yuan;ZHOU Li;HAO Qing-xiu;YANG Jian;HUANG Lu-qi(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences/State Key Laboratory Breeding Base of Dao-di Herbs,Beijing 100700,China;College of Pharmacy,Ningxia Medical University,Yinchuan 750004,China;Ningbo Zhongsheng Product Testing Co.,Ltd.,Ningbo 315000,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]中国中医科学院中药资源中心/道地药材国家重点实验室培育基地,北京100700 [3]宁夏医科大学药学院,宁夏银川750004 [4]宁波中盛产品检测有限公司,浙江宁波315000 [5]中国中医科学院,北京100700

出　　处：《中药材》2023年第9期2224-2232,共9页Journal of Chinese Medicinal Materials

基　　金：山东省重点研发计划(重大科技创新工程)项目(2021CXGC010508);国家产业技术基础公共服务平台项目(2022-230-221);中国中医科学院科技创新工程项目(CI2021A04005)。

摘　　要：目的:通过测定不同产地丹参中40个无机元素含量,研究元素分布差异,并对重金属及有害元素进行健康风险评估。方法:收集来自5个丹参主产区的31批467份样品,采用ICP-MS法测定其中无机元素的含量,利用化学计量学方法进行产地鉴别分析和差异元素筛选,通过计算重金属及有害元素的单项污染指数、内梅罗综合污染指数对其进行安全性评价,并根据每日最大耐受摄入量和致癌风险进行健康风险评估。结果:四川丹参的稀土元素La、Ce含量最高,山东丹参的无机元素Na、Sr、Nb和Cu的含量最高,其余元素以河南和陕西丹参的含量相对较高。不同产地丹参无机元素的含量具有显著差异,线性判别分析模型对丹参元素产地的判别效果最佳,其训练集和预测集准确率均达到90%以上。安全性评价表明大部分样品污染状况均处于安全状态。健康风险评估模型结果显示使用丹参无明显健康风险,但个别基地的As暴露引起的致癌性健康风险需要被关注。结论:不同产地丹参无机元素分布具有特征性,可利用这些特征进行产地鉴别。丹参药材的重金属含量均处于安全水平,并无明显健康风险。本研究为大宗药材丹参溯源检测体系的构建及健康风险评估和质量评价体系建立提供了参考。Objective:To study the distribution differences of 40 inorganic elements in Salviae Miltiorrhizae Radix et Rhizoma from different producing areas,and to evaluate the health risks of heavy metals and harmful elements.Methods:467 samples of 31 batches from 5 major producing areas of Salviae Miltiorrhizae Radix et Rhizoma were collected,and the contents of inorganic elements were determined by ICP-MS method.The origin identification analysis and different elements screening were carried out by stoichiometric method.The safety evaluation was carried out by calculating the single pollution index and Nemerow comprehensive pollution index of heavy metals and harmful elements.Health risks were assessed based on maximum tolerable daily intake and cancer risk.Results:The content of rare earth elements La and Ce in Salviae Miltiorrhizae Radix et Rhizoma from Sichuan was the highest,the content of inorganic elements Na,Sr,Nb and Cu in Salviae Miltiorrhizae Radix et Rhizoma from Shandong was the highest,and the content of other elements in Salviae Miltiorrhizae Radix et Rhizoma from Henan and Shaanxi was relatively high.There were significant differences in the content of inorganic elements of Salviae Miltiorrhizae Radix et Rhizoma from different habitats.The linear discriminant analysis model had the best discriminant effect on the origin of Salviae Miltiorrhizae Radix et Rhizoma,and the accuracy of its training set and prediction set both reached more than 90%.Safety evaluation showed that most samples were in safe state.The results of the health risk assessment model showed that there was no obvious health risk from the use of Salviae Miltiorrhizae Radix et Rhizoma,but the carcinogenic health risk caused by As exposure in individual bases should be paid attention to.Conclusion:The distribution of inorganic elements of Salviae Miltiorrhizae Radix et Rhizoma from different habitats has characteristic characteristics.The content of heavy metals in Salviae Miltiorrhizae Radix et Rhizoma is at the safe level and has no obvious

关 键 词：丹参 ICP-MS 化学计量学 无机元素 健康风险评估 

分 类 号：R284.1[医药卫生—中药学]