东北地区野生与栽培五味子质量评价研究  

Quality Evaluation of Wild and Cultivated Schisandrae Chinensis Fructus in Northeast China

作　　者：宋天宝 弓思涵 葛珈铭 韩翠婷 李佳荣 付鲲[2] 李先宽[1] SONG Tian-bao;GONG Si-han;GE Jia-ming;HAN Cui-ting;LI Jia-rong;FU Kun;LI Xian-kuan(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Second Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300150,China)

机构地区：[1]天津中医药大学,天津301617 [2]天津中医药大学第二附属医院,天津300150

出　　处：《中药材》2023年第9期2233-2239,共7页Journal of Chinese Medicinal Materials

基　　金：天津市教委科研计划项目(2018KJ030)。

摘　　要：目的:对东北地区野生与栽培五味子质量进行评价,以期为五味子优良种质筛选提供依据。方法:采用UPLC建立五味子的指纹图谱及含量测定方法,并对数据进行单因素方差分析及化学模式识别分析。结果:39批五味子样品指纹图谱有22个共有峰,指认出7成分,分别为五味子醇甲、五味子醇乙、五味子酯甲、五味子酚、五味子甲素、五味子乙素、五味子丙素。野生、栽培五味子中上述7个成分的总含量分别为0.8565%~1.7909%、0.8901%~1.8184%,野生品中S14号样品的总含量最高,栽培品中S31号样品的总含量最高;通过主成分分析和正交偏最小二乘判别分析筛选出五味子野生与栽培品的11个差异性成分,五味子丙素含量差异最为显著。结论:以木脂素类成分为评价指标,东北地区野生与栽培五味子药材质量存在差异,但7个成分总含量无显著差异。野生品S2、S9、S14号及栽培品S19、S31号可进一步用于开展优良种质筛选研究。Objective:To evaluate the quality of wild and cultivated Schisandrae Chinensis Fructus in northeast China,so as to provide evidence for the selection of fine germplasm of Schisandrae Chinensis Fructus.Methods:UPLC was used to establish the method of fingerprint and content determination of Schisandrae Chinensis Fructus,and the data were analyzed by single factor variance analysis and chemical pattern recognition analysis.Results:There were 22 common peaks in the fingerprints of 39 batches of Schisandrae Chinensis Fructus,and 7 components were identified,which were schizandrol A,schizandrol B,schisantherin A,schisanhenol,schisandrin A,schisandrin B and schisandrin C.The total contents of these seven components in wild and cultivated Schisandrae Chinensis Fructus were 0.8565%to 1.7909%and 0.8901%to 1.8184%,respectively.The total contents of S14 wild sample and S31 cultivated sample were the highest,respectively.By principal component analysis and orthogonal partial least square discriminant analysis,11 different components were found in wild and cultivated Schisandrae Chinensis Fructus,and the difference of schisandrin C content was the most significant.Conclusion:Taking lignan composition as evaluation index,there is difference in quality between wild and cultivated Schisandrae Chinensis Fructus in northeast China,but there is no significant difference in total content of 7 components.S2,S9,S14 wild samples and S19,S31 cultivated samples can be further used for screening of superior germplasm.

关 键 词：五味子 UPLC 指纹图谱 化学模式识别 种质 

分 类 号：R284.1[医药卫生—中药学]