不同激素配比对小草海桐组培技术的影响研究  

Study on the Influence of Different Hormone Ratio on Tissue Culture Technology of Scaevola hainanensis

作　　者：高伟勤 王佳 童升洪 赵秀芳 Gao Weiqin;Wang Jia;Tong Shenghong;Zhao Xiufang(Lingnan Eco-Cultural Tourism Co.,Ltd.,Dongguan 523125,Guangdong,China)

机构地区：[1]岭南生态文旅股份有限公司,广东东莞523125

出　　处：《农业技术与装备》2024年第3期119-121,124,共4页Agricultural Technology & Equipment

基　　金：东莞市社会发展科技项目(20221800905472);岭南股份科技创新项目(KY-0093)。

摘　　要：以半红树植物小草海桐叶片为外植体,MS为基础培养基,设计不同质量浓度浓度的6-BA、NAA和6-BA等激素,探究不同阶段小草海桐组培技术的最适宜浓度。结果表明,愈伤组织诱导阶段MS+0.5 mg/L NAA+0.1 mg/L 6-BA的培养基效果最好,诱导率为82.01%;在不定芽的诱导阶段,MS+1.0 mg/L 6-BA的培养基效果最好,出芽率为60.42%;在生根培养过程中,MS+0.5 mg/L IAA的培养基效果最好,生根率为87.67%。Using the leaves of the semi-mangrove plant Scaevola hainanensis as explants and MS as the base medium,different concentrations of hormones such as 6-BA,NAA and 6-BA were designed to explore the optimal concentration of tissue culture technology of Scaevola hainanensis in different stages.The results showed that MS+0.5 mg/L NAA+0.1 mg/L6-BA medium had the best effect on callus induction stage,and the induction rate was 82.01%.In the stage of bud induction,MS+1.0 mg/L6-BA medium had the best effect,and the budding rate was 60.42%.In the process of rooting culture,MS+0.5 mg/L IAA medium had the best effect,and the rooting rate was 87.67%.

关 键 词：半红树 小草海桐 组织培养 激素 

分 类 号：S532[农业科学—作物学]