蒙药复方沙棘颗粒剂中甘草酸单铵盐、总黄酮的含量测定  

Determination of Monoammonium Glycyrrhizinate and Total Flavonoids in Compound Shaji Granules

作　　者：王海荣 孟柯 特格喜白音 赵岩 乌日娜 WANG Hairong;MENG Ke;Tegexibaiyin;ZHAO Yan;Wurina(Inner Mongolia Traditional Chinese and Mongolian Medical Research Institute,Hohhot 010010,China;Ordos Institute of Mongolian Medicine,Ordos 017010,China;Affiliated Hospital of Inner Mongolia University for the Nationalities,Tongliao 028007,China;Inner Mongolia International Mongolian Hospital,Hohhot 010065,China)

机构地区：[1]内蒙古自治区中蒙医药研究院,内蒙古呼和浩特010010 [2]鄂尔多斯市蒙医研究所,内蒙古鄂尔多斯017010 [3]内蒙古民族大学附属医院,内蒙古通辽028007 [4]内蒙古自治区国际蒙医医院,内蒙古呼和浩特010065

出　　处：《中国民族民间医药》2024年第7期37-40,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：2017年内蒙古自治区科技计划项目“蒙药复方沙棘颗粒剂新药研究”。

摘　　要：目的:建立蒙药复方沙棘颗粒剂中甘草酸单铵盐、总黄酮的含量测定方法。方法:采用高效液相色谱法,C_(18)(250 mm×4.6 mm,5μm)为色谱柱,以乙腈-0.05%磷酸溶液(38∶62)为流动相,体积流量为1.0 mL/min,进样量为10μL,在252 nm波长下测定甘草酸单铵盐含量;利用紫外可见分光光度法,以芦丁为对照品,在510 nm波长下检测蒙药复方沙棘颗粒剂总黄酮含量。结果:甘草酸单铵盐对照品在0.053~0.530μg范围内与峰面积呈良好的线性关系,Y=11.003X+0.0031,r=1,平均回收率为105.9%,RSD=1.22%(n=6);芦丁对照品在2.0~80.0μg/mL范围内与吸光度值呈良好的线性关系,线性回归方程为Y=11.537X+0.0014,r=0.9999,平均回收率为99.20%,RSD值为0.54%(n=6)。结论:该方法操作简便,准确,具有良好的精密度、重复性和稳定性,适用于蒙药复方沙棘颗粒剂的质量控制。Objective To establish a method for the content determination of monoammonium glycyrrhizinate and total flavonoids in compound Shaji granules.Methods The content of monoammonium glycyrrhizinate was determined by HPLC with C_(18)(250 mm×4.6 mm,5μm)was used as chromatographic column,acetonitrile-0.05%phosphoric acid(38∶62)as mobile phase,volume flow rate was 1.0 mL/min,injection volume was 10μL and wavelength was 252 nm.The total flavonoids was determined at 510 nm by UV with rutin as the control.Results Monoammonium glycyrrhizinate had a good linear relationshipin the range of 0.053-0.530μg.The linear regression equation was Y=11.003X+0.0031,r=1.The average recovery was 105.9%,RSD=1.22%(n=6).The rutin reference substance showed a good linear relationship with the absorbance value in the range of 2.0-80.0μg/mL.The linear regression equation was Y=11.537X+0.0014,r=0.9999.The average recovery was 99.20%,RSD=0.54%(n=6).Conclusion The method is simple,accurate,with good precision,repeatability and stability.It is suitable for the quality control of compound Shaji granules.

关 键 词：蒙药复方沙棘颗粒剂 甘草酸单铵盐 总黄酮 含量测定 质量标准 

分 类 号：R29[医药卫生—民族医学]