制首乌不同配伍方式对黑色素及酪氨酸酶活性的影响  

Effect of Different Compatibility Methods of Radix Polygoni Multiflori Preparata on Melanin and Tyrosinase Activity

作　　者：缪源 MIAO Yuan(Department of Traditional Chinese Medicine,Yongdeng County People’s Hospital,Lanzhou 730300,China)

机构地区：[1]永登县人民医院中医科,甘肃兰州730300

出　　处：《基层中医药》2024年第3期114-118,共5页Basic Traditional Chinese Medicine

摘　　要：目的观察制首乌不同配伍方式的水提物对黑色素上调率及酪氨酸酶活性的影响,优选制首乌“乌须发”最佳的配伍方式。方法分别采用蘑菇酪氨酸酶NaOH裂解法及多巴胺速率氧化法研究制首乌不同配伍方式对黑色素生物合成及酪氨酸酶活性的影响。结果制首乌不同配伍方式均具有黑色素上调的作用,整体表现为制首乌-黑芝麻>制首乌-侧柏叶>制首乌-桑椹>制首乌-女贞子,且在一定范围内呈一定的剂量依赖性;制首乌不同配伍方式对酪氨酸酶的激活率均不同,其中制首乌-侧柏叶和制首乌-桑椹对酪氨酸酶具有抑制作用,但是随着浓度的增加,抑制作用逐渐减弱;制首乌-黑芝麻和制首乌-女贞子对酪氨酸酶具有激活作用,制首乌-黑芝麻较制首乌-女贞子激活作用明显。结论制首乌-黑芝麻为制首乌“乌须发”的最佳配伍,制首乌-黑芝麻配伍能够激活酪氨酸酶的活性,上调黑色素含量。Objective To observe the effect of water extracts from different compatibility methods of Radix Polygoni Multiflori Preparata on the increasing rate of melanin and tyrosinase activity and select the best compatibility method of Radix Polygoni Multiflori Preparata for improving hair quality.Methods The effects of different compatibility methods of Radix Polygoni Multiflori Preparata on melanin biosynthesis and tyrosinase activity were studied by using the NaOH lysis method of mushroom tyrosinase and dopamine rate oxidation method,respectively.Results Different compatibility methods of Radix Polygoni Multiflori Preparata increased melanin,with the overall performance being Radix Polygoni Multiflori Preparata-Sesamum indicum L.>Radix Polygoni Multiflori Preparata-Cacumen biotae>Radix Polygoni Multiflori Preparata-Morus alba L.>Radix Polygoni Multiflori Preparata-Ligustrum lucidum Ait.,and there was a certain dose dependence within a certain range.The activation rate of tyrosinase varied with different compatibility methods of Radix Polygoni Multiflori Preparata.Among them,Radix Polygoni Multiflori Preparata-Cacumen biotae and Radix Polygoni Multiflori Preparata-Morus alba L.had inhibitory effects on tyrosinase,but the inhibitory effect gradually weakened with increasing concentration.Radix Polygoni Multiflori Preparata-Sesamum indicum L.and Radix Polygoni Multiflori Preparata-Ligustrum lucidum Ait.had an activating effect on tyrosinase,and Radix Polygoni Multiflori Preparata-Sesamum indicum L.had a more significant activating effect than Radix Polygoni Multiflori Preparata-Ligustrum lucidum Ait..Conclusion Radix Polygoni Multiflori Preparata-Sesamum indicum L.is the best compatibility method of Radix Polygoni Multiflori Preparata for improving hair quality,which can activate the activity of tyrosinase and increase the content of melanin.

关 键 词：制首乌 配伍 黑色素 酪氨酸酶 

分 类 号：R28[医药卫生—中药学]