A Rapid Parameter of Enzyme-Treated Cellulosic Material Revealed by Reducing Sugar Release  

作　　者：Verônica Távilla Ferreira Silva Adriane Maria Ferreira Milagres 

机构地区：[1]Departamento de Biotecnologia,Escola de Engenharia de Lorena,Universidade de São Paulo,Lorena,SP,12602-810,Brazil

出　　处：《Journal of Renewable Materials》2024年第3期539-551,共13页可再生材料杂志（英文）

基　　金：supported by CNPq(303416/2018-1)and FAPESP(2019/25867-3).

摘　　要：This study was conducted to evaluate the effectiveness of enzymes in purifying and reducing the degree of polymerization of cellulose for the production of dissolving pulp.Our goal was to determine the contributions of xylanase(X)and endoglucanase(EG)in the treatment of pulp,specifically by quantifying the formation of soluble and insoluble reducing sugars using the dinitrosalycilic acid(DNS)test.Predominantly,the release of soluble reducing sugars(RSSol)was enhanced after xylanase treatment,while endoglucanase(EG)treatment led to changes in insoluble reducing sugars(RSIns).The maximum synergism was observed for RSIns when a high ratio of endoglucanase to xylanase(320EG:5X/g pulp)was used.The relative contribution of endoglucanase to RSins was determined to be 15.6%of the total reducing sugar.The viscosity of pulps treated with xylanase decreased only by 7%,whereas endoglucanase treatment significantly reduced viscosity by 45%.Modifications in the particle size were observed after pulp treatment with the combination of endoglucanase and xylanase.In summary,the DNS test is a rapid and effective method for evaluating the efficiency of enzyme treatments on pulps.The measurement of RSIns correlates with changes in pulp viscosity to different extents,providing valuable insights into the effectiveness of enzyme treatments.

关 键 词：ENDOGLUCANASE XYLANASE cellulose fiber PULP synergism 

分 类 号：TB30[一般工业技术—材料科学与工程]