小鼠卵母细胞减数分裂中心粒蛋白SAS-6的表达和亚细胞分布  

Protein expression and subcellular distribution of centriolar component SAS-6 in mouse oocyte meiosis

作　　者：宋可 宋柯 李静宇 马伟[2] 杨晓葵[1] SONG Ke;SONG Ke;LI Jingyu;MA Wei;YANG Xiaokui(Department of Human Reproductive Medicine,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing Maternal and Child Health Care Hospital,Beijing 100026;Department of Histology and Embryology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学附属北京妇产医院/北京妇幼保健院生殖医学科,北京100026 [2]首都医科大学基础医学院组织学与胚胎学教研室,北京100069

出　　处：《基础医学与临床》2024年第5期651-657,共7页Basic and Clinical Medicine

基　　金：北京市自然科学基金(7242003);北京市医院管理中心“登峰”计划(DFL20191401)。

摘　　要：目的研究在小鼠卵母细胞减数分裂成熟过程中,中心粒蛋白SAS-6的蛋白表达模式和亚细胞定位分布。方法采用免疫荧光染色方法分析SAS-6在中国仓鼠卵巢细胞系(CHO细胞)有丝分裂过程中和卵母细胞减数分裂过程中的亚细胞分布,及其与微管组织中心(MTOCs)和囊泡之间的相关性;通过Western blot测定SAS-6在小鼠卵母细胞减数分裂各阶段的蛋白质表达水平。结果在体细胞有丝分裂过程中SAS-6始终与中心粒周围蛋白Pericentrin保持共定位;在小鼠卵母细胞内SAS-6恒定表达于减数分裂的各个阶段并特异性聚集在高尔基体基质蛋白GM130阳性的囊泡上,而没有分布在MTOCs上。结论SAS-6可能在卵母细胞减数分裂过程中通过调节囊泡而参与纺锤体的趋皮质区迁移。Objective To study the expression and subcellular distribution pattern of centriolar protein SAS-6 during meiosis of mouse oocytes.Methods Immunefluorescence was conducted to analyze the subcellular distribution pattern of SAS-6 in Chinese hamster ovary cell line(CHO cell)during mitosis.Western blot was applied to detect the protein expression of SAS-6 in mouse oocyte meiosis.Immunofluorescence microscopy carried out to determine the subcellular distribution of SAS-6 and its association with microtubule organizing centers(MTOCs)and in vesicles during the oocyte meiosis.Results SAS-6 was colocalized with Pericentrin on spindle poles during the mitotic process in somatic cells.In mouse oocytes,SAS-6 was stably and consistently expressed at all stages of meiosis and specifically aggregated on GM130-positive vesicles,but not in MTOCs.The results suggested that centriolar protein SAS-6 was still expressed in oocytes lacking centrosomes,and localized in GM130-positive vesicles but expressed on percientrin-positive MTOCs.Conclusions SAS-6 may play a role in spindle cortical migration through regulating vesicles during oocyte meiosis.

关 键 词：纺锤体异常组装蛋白6 卵母细胞 微管组织中心 囊泡 纺锤体迁移 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]