沙漠干热环境下大鼠运动骨骼肌损伤评价指标的筛选  

Screening of evaluation indicators for motor skeletal muscle injury in rats in hot and dry desert environment

作　　者：武堂晓 杨曦[2] 尹紫依 吴荧秋 吴吉利 惠华强[3] WU Tangxiao;YANG Xi;YIN Ziyi;WU Yingqiu;WU Jili;HUI Huaqiang(School of Public Health,Xinjiang Medical University,Urumqi,Xinjiang Uygur Autonomous Region,830011;Research and teaching department,General Hospital of Xinjiang Military Command,Urumqi,Xinjiang Uygur Autonomous Region,830000;health service department,General Hospital of Xinjiang Military Command,Urumqi,Xinjiang Uygur Autonomous Region,830000;Faculty of Preventive Medicine,Shihezi University School of Medicine,Shihezi,Xinjiang Uygur Autonomous Region,832061,China)

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐市830011 [2]新疆军区总医院科研教学科,乌鲁木齐市830000 [3]新疆军区总医院卫勤部,乌鲁木齐市830000 [4]石河子大学医学院预防医学系,新疆维吾尔自治区石河子市832061

出　　处：《陆军军医大学学报》2024年第8期894-900,共7页Journal of Army Medical University

基　　金：自治区区域协同创新专项(科技援疆计划,2022E02121);新疆维吾尔自治区研究生科研创新项目资助(XJ2023G190)。

摘　　要：目的使用西北特殊环境人工实验舱建立沙漠干热环境大鼠运动骨骼肌损伤模型,探讨沙漠干热环境下大鼠运动骨骼肌损伤的组织形态学和骨骼肌损伤相关等指标,筛选干热环境运动骨骼肌损伤评价指标。方法18只8周龄雄性SD大鼠按随机数字表法分为(n=6):空白对照组(常温环境中自由运动)、常温运动组(常温环境中力竭运动)、干热运动组(干热环境中力竭运动)。3组大鼠先进行3 d的运动跑台适应性训练(速度为16 m/min,坡度为-16°,时间为5~10 min);常温运动组和干热运动组大鼠以19.3 m/min速度、-16°坡度运动至力竭结束,1次/d;干热运动组采用西北特殊环境人工实验舱模拟沙漠干热环境[温度(40±1)℃,湿度(10±2)%]建立模型。实验连续6 d,取大鼠比目鱼肌组织观察病理学变化,通过血清学检测大鼠骨骼肌损伤标志物、炎症因子、氧化应激、激素等相关指标。结果干热运动组骨骼肌损伤标志物肌酸激酶(concentrations of creatine kinase,CK)、肌酸激酶同工酶(creatine kinase isoenzyme,CK-MB)、肌型肌酸激酶(muscle-specific creatine kinase,CK-MM)的浓度均高于空白对照组、常温运动组(P<0.05);干热运动组炎症因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-1β(interleukin-1β,IL-1β)、IL-6的浓度均高于空白对照组、常温运动组(P<0.05);干热运动组氧化应激丙二醛(malondialdehyde,MDA)的浓度均高于空白对照组、常温运动组(P<0.05);干热运动组超氧化物歧化酶(superoxide dismutase,SOD)的浓度低于空白对照组、常温运动组(P<0.05);干热运动组睾酮(testosterone,T)的浓度均低于空白对照组、常温运动组(P<0.05);干热运动组皮质酮(corticosterone,Cort)的浓度高于空白对照组、常温运动组(P<0.05);干热运动组睾酮/皮质酮(testosterone/corticosterone,T/Cort)的浓度低于空白对照组、常温运动组(P<0.05);骨骼肌损伤标志物CK、CK-MB和CK-MM与促炎症�Objective To explore the histomorphology and related indicators in a rat model of skeletal muscle injury in an artificial experimental chamber as special environment in Northwest China to stimulate a hot and dry desert environment in order to screen the evaluation indicators of skeletal muscle injury in a hot and dry environment.Methods Eighteen male SD rats(8 weeks old)were randomly divided into blank control group(room temperature,free movement),room temperature exercise group,and hot and dry exercise group,with 6 rats in each group.After the rats were subjected to adaptation training for 3 d(a 3-D exercise treadmill,speed:16 m/min,slope:-16°,time:5~10 min),the rats in the 2 exercise groups were subjected to a treadmill once per day at a speed of 19.3 m/min and a slope of-16°till the end of exhaustion,and those in the hot and dry exercise group were housed in an artificial experimental chamber at a temperature of(40±1)°C,a humidity of(10±2)%.After 6 d of continuous exercise,the rat soleus muscle tissue was taken to observe the pathological changes,and the serum samples were detected for skeletal muscle injury markers,inflammatory factors,oxidative stress related factors,hormones and other related indicators.Results When compared with the blank control group and the room temperature exercise group,the hot and dry exercise group had significantly higher concentrations of creatine kinase(CK),creatine kinase isoenzyme(CK-MB)and muscle-specific creatine kinase(CK-MM)(P<0.05),elevated contents of TNF-α,IL-1βand IL-6(P<0.05),raised malondialdehyde(MDA)content and lower superoxide dismutase(SOD)activity(P<0.05),lower testosterone(T)but higher corticosterone(Cort)levels and thus lower testosterone/corticosterone(T/Cort)ratio(P<0.05).The markers of skeletal muscle injury,CK,CK-MB and CK-MM were positively correlated with the pro-inflammatory cytokines,TNF-α,IL-1βand IL-6(P<0.05),positively with the antioxidant indicator MDA but negatively with SOD(P<0.05),and positively correlated with Cort level and negatively

关 键 词：沙漠干热环境 运动 骨骼肌损伤 肌酸激酶 炎症因子 氧化应激 

分 类 号：R873.4[医药卫生—运动医学] R122.21[医药卫生—临床医学] R122.23R685.4