丁酸钠对低氧寒冷暴露大鼠的保护效应研究  

Protective effect of sodium butyrate on hypoxia tolerance in rats exposed to hypoxia and cold

作　　者：郭晓玉 谭小玲[2,3] 崔琪 谢宏晨 黄榆杰 孟祥琼 蒋雯俊 丁宇 荆海霞[1] GUO Xiaoyu;TAN Xiaoling;CUI Qi;XIE Hongchen;HUANG Yujie;MENG Xiangqiong;JIANG Wenjun;DING Yu;JING Haixia(College of Agriculture and Animal Husbandry,Qinghai University,Xining,Qinghai Hui Autonomous Region,810016;Department of Cold Region Medicine,College of Plateau Military Medicine,Army Medical University(Third Military Medical University),Chongqing,400038,China;Key Laboratory of Extreme Environmental Medicine of Ministry of Education,College of Plateau Military Medicine,Army Medical University(Third Military Medical University),Chongqing,400038,China)

机构地区：[1]青海大学农牧学院动物医学系,西宁810016 [2]陆军军医大学(第三军医大学)高原军事医学系寒区医学教研室,重庆400038 [3]陆军军医大学(第三军医大学)高原军事医学系极端环境医学教育部重点实验室,重庆400038

出　　处：《陆军军医大学学报》2024年第8期901-911,共11页Journal of Army Medical University

摘　　要：目的探究丁酸钠对低氧寒冷暴露下大鼠的保护作用及其机制。方法7~8周、体质量240~260 g雄性SD大鼠随机分为:常氧常温生理盐水对照(normoxia normothermia saline control,NNC,n=10)组、常氧常温丁酸钠(normoxia normothermia sodium butyrate,NNB,n=10)组、低氧寒冷生理盐水对照(hypoxia cold saline control,HCC,n=19)组和低氧寒冷丁酸钠(hypoxia cold sodium butyrate,HCB,n=19)组。NNB、HCB组的灌胃剂量为200 mg/kg,NNC、HCC组的灌胃剂量为5 mL/kg。①连续灌胃7 d后,HCC、HCB组大鼠置于低压低氧舱内模拟海拔5000 m、温度8℃暴露7 d,取腹主动脉血进行血气、血常规和血清生化指标检测,ELISA测定血清炎症因子和内分泌激素。NNC、NNB组大鼠在舱外同时喂养,7 d后进行指标检测,评价丁酸对机体的保护效应(n=10)。②监测HCC、HCB组大鼠核心体温,评价丁酸对低氧寒冷暴露大鼠的体热平衡(n=3)。③检测低氧寒冷暴露前后HCC、HCB大鼠的低氧(平原低氧箱,11%O_(2))运动耐力,评价耐缺氧能力(n=6)。结果与NNC组比较,HCC组大鼠动脉血氧饱和度(arterial oxygen saturation,SaO_(2))、动脉血氧分压(arterial oxygen partial pressure,PaO_(2))、IL-4、内分泌激素雌二醇(estradiol,E_(2))、皮质醇(cortisol,F)、核心体温以及低氧暴露下的运动时长显著降低(P<0.05);而红细胞数目(red blood cell,RBC)、血红蛋白浓度(haemoglobin,HGB)、红细胞压积(hematocrit,HCT)、血清心肌钙蛋白(cardiac troponin,CRDAC-T)、尿酸(uric acid,UA)、谷丙转氨酶(alanine aminotransferase,ALT)、总胆固醇(total cholesterol,TCH)和低密度脂蛋白(low-density lipoprotein,LDL)、IL-6以及粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony-stimulating factor,GM-CSF)显著升高,差异具有统计学意义(P<0.05)。与HCC组比较,HCB组大鼠动脉SaO_(2)、PaO_(2)、IL-4、E_(2)、F、促肾上皮质激素释放激素(corticotropin releasing hormone,CRH)、促肾上腺皮质激素(adrenocorticotrObjective To investigate the protective effects and underlying mechanisms of sodium butyrate on rats exposed to hypoxia and cold conditions.Methods Fifty-eight male SD rats(aged 7~8 weeks,weighing 240~260 g)were randomly divided into normoxia normothermia saline control(NNC)group(n=10),normoxia normothermia sodium butyrate(NNB)group(n=10),hypoxia cold saline control(HCC)group(n=19),and hypoxia cold sodium butyrate(HCB)group(n=19).The intragastric dose of sodium butyrate was 200 mg/kg for the NNB and HCB groups,while the NNC and HCC groups were given normal saline of 5 mL/kg.①After continuous intragastric administration for 7 d,the rats in the HCC and HCB groups were placed in a low-pressure hypoxic chamber to simulate an altitude of 5000 m and exposed to a temperature of 8℃for 7 d.Subsequently,blood samples were collected from the abdominal aorta for blood gas analysis,blood routine test,and detection for serum biochemical indicators.ELISA was used to determine serum inflammatory cytokines and endocrine hormones.The rats in the NNC and NNB groups(n=10)were fed outside the chamber and underwent the same tests in 7 d later to evaluate the protective effects of sodium butyrate on the body.②Core body temperature monitoring was conducted to assess the impact of sodium butyrate on thermoregulation in rats exposed to hypoxia and cold(n=3).③Hypoxia exercise tolerance of the HCC group and HCB group in a hypoxic chamber(11%O_(2))was evaluated for their hypoxia resistance(n=6).Results Compared to the NNC group,the rats in the HCC group exhibited significant decreases in arterial oxygen saturation(SaO_(2))and arterial oxygen partial pressure(PaO_(2)),serum levels of IL-4,estradiol(E_(2))and cortisol(F),core temperature,and exercise duration(P<0.05),and had notably increased levels of red blood cell(RBC)count,hemoglobin(HGB),hematocrit(HCT),cardiac troponin(CRDAC-T),uric acid(UA),alanine aminotransferase(ALT),total cholesterol(TC),low-density lipoprotein(LDL),IL-6 and granulocyte-macrophage colony-stimulating factor(G

关 键 词：丁酸钠 习服 低氧 寒冷 耐缺氧 

分 类 号：R-332[医药卫生] R339.55R977.6