SiRAP2-12,a Positive Regulatory Factor,Effectively Improves the Waterlogging Tolerance of Foxtail Millet(Setaria italica)  

作　　者：Xueyan Xia Xiaohong Fu Yu Zhao Jihan Cui Nuoya Xiao Jingxin Wang Yiwei Lu Meihong Huang Cheng Chu Jia Zhang Mengxin Yang Shunguo Li Jianfeng Liu 

机构地区：[1]Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals(Co-Construction byMinistry and Province),Ministry of Agriculture and Rural Affairs/The Key Research Laboratory of Minor Cereal Crops of Hebei Province,Shijiazhuang,050035,China [2]School of Life Sciences,Institute of Life Science and Green Development,Hebei University,Baoding,071002,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第3期445-465,共21页国际实验植物学杂志（英文）

基　　金：This research was supported by the China Agricultural Research System(CARS-06-14.5-A23);HAAFS Basic Science and Technology Contract Project(Grant No.HBNKY-BGZ-02);Technical System of Foxtail Millet Industry in Hebei Province.

摘　　要：Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield reduc-tion.ERF family plays an important role to plant adversity tolerance.In our study,we obtained 19,819 differential expressed genes(DEGs)between the two treatments based on the RNA-seq sequencing of foxtail millet of water-logging stress.Furthermore,a total of 28 ERF family members were obtained,which have a complete open read-ing frame.We studied the evolution and function of SiERF family and how they affected the waterlogging tolerance.It was found that SiERF1A/B/C(GenBank ID:OR775217,OR775219,OR775218)and SiRAP2-12(GenBank ID:OR775216)have similar functions to the known waterlogging tolerance genes of other plants.Among them,the SiRAP2-12 expression was obviously significantly up-regulated in foxtail millet after 5d water-logging stress.After SiRAP2-12 was silenced,the activity of defense enzymes in millet decreased significantly.In details,superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),the osmotic regulator proline(Pro),and the activity of the anaerobic respiratory enzyme alcohol dehydrogenase(ADH)content were decreased by 78.61%,29.52%,79.95%,19.41%and 54.77%,respectively.In contrast,the relative electrical conductivity contents(REC),malondialdehyde(MDA),and hydrogen peroxide(H_(2)O_(2))of the foxtail millet subjected to virus-induced gene silencing clearly increased by 1.03-fold,36.09%,and 15.21%,respectively.The content of sodium(Na^(+))in the SiRAP2-12-silenced foxtail millet also increased,but that of potassium(K^(+))decreased.Interestingly,we found that ethylene content was significantly reduced.Further,the SiAOC1 expression,an essential gene for ethylene synthesis,was inhibited in SiRAP2-12-silenced foxtail millet after waterlogging stress.Taken together,we hypothesized that SiRAP2-12 might be a positive regulator of millet tolerance to waterlogging stress.

关 键 词：Foxtail millet WATERLOGGING SiRAP2-12 VIGS ETHYLENE 

分 类 号：Q94[生物学—植物学]