Genome-Wide Exploration of the Grape GLR Gene Family and Differential Responses of VvGLR3.1 and VvGLR3.2 to Low Temperature and Salt Stress  

作　　者：Honghui Sun Ruichao Liu Yueting Qi Hongsheng Gao Xueting Wang Ning Jiang Xiaotong Guo Hongxia Zhang Chunyan Yu 

机构地区：[1]The Engineering Research Institute of Agriculture and Forestry,Ludong University,Yantai,264025,China [2]College of Agriculture,Ludong University,Yantai,264025,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第3期533-549,共17页国际实验植物学杂志（英文）

基　　金：This research was funded by the Natural Science Foundation of Shandong Province of China(ZR2022MC144).

摘　　要：Grapes,one of the oldest tree species globally,are rich in vitamins.However,environmental conditions such as low temperature and soil salinization significantly affect grape yield and quality.The glutamate receptor(GLR)family,comprising highly conserved ligand-gated ion channels,regulates plant growth and development in response to stress.In this study,11 members of the VvGLR gene family in grapes were identified using whole-genome sequence analysis.Bioinformatic methods were employed to analyze the basic physical and chemical properties,phylogenetic trees,conserved domains,motifs,expression patterns,and evolutionary relationships.Phylogenetic and collinear analyses revealed that the VvGLRs were divided into three subgroups,showing the high conservation of the grape GLR family.These members exhibited 2 glutamate receptor binding regions(GABAb and GluR)and 3-4 transmembrane regions(M1,M2,M3,and M4).Real-time quantitative PCR analysis demonstrated the sensitivity of all VvGLRs to low temperature and salt stress.Subsequent localization studies in Nicotiana tabacum verified that VvGLR3.1 and VvGLR3.2 proteins were located on the cell membrane and cell nucleus.Additionally,yeast transformation experiments confirmed the functionality of VvGLR3.1 and VvGLR3.2 in response to low temperature and salt stress.Thesefindings highlight the significant role of the GLR family,a highly conserved group of ion channels,in enhancing grape stress resistance.This study offers new insights into the grape GLR gene family,providing fundamental knowledge for further functional analysis and breeding of stress-resistant grapevines.

关 键 词：Genome-wide identification glutamate receptor(GLR)family low temperature stress salt stress GRAPE 

分 类 号：Q94[生物学—植物学]