松萝酸抑制人膀胱癌细胞增殖作用机制研究  

Inhibitory Effect of Usnic Acid on Proliferation of Human Bladder Cancer Cells

作　　者：曹鹤 史丽颖[1] 郭秀磊 于大永[1] CAO He;SHI Liying;GUO Xiulei;YU Dayong(School of Life Science and Technology,Dalian University,Dalian 116622,China)

机构地区：[1]大连大学生命科学与技术学院,大连116622

出　　处：《华南师范大学学报（自然科学版）》2024年第1期72-77,共6页Journal of South China Normal University(Natural Science Edition)

基　　金：辽宁省科学技术计划项目(2019-ZD-0564)。

摘　　要：为了探究松萝酸对人膀胱癌的影响,体外培养人膀胱癌细胞T24、RT4,用不同浓度(12.5、25、50、100、200μmol/L)的松萝酸分别处理T24、RT4细胞24 h和48 h,采用MTT法检测T24、RT4细胞的增殖情况。研究结果发现松萝酸处理后细胞内的活性氧(ROS)水平增加且通过线粒体途径、死亡受体途径来诱导T24细胞发生凋亡:(1)松萝酸对T24、RT4细胞增殖均有抑制作用,且均呈现时间和剂量依赖性,其中,松萝酸对T24细胞增殖的抑制率更高;(2)通过Hoechst 33342染色与Annexin V-FITC/PI双染发现,随着松萝酸浓度的升高,凋亡的T24细胞逐渐增多;(3)T24细胞经松萝酸处理后,细胞内ROS水平升高,cleaved-Caspase-3、cleaved-Caspase-8、cleaved-Caspase-9和Bax蛋白的表达量增加,Caspase-3、Caspase-8、Caspase-9和Bcl-2蛋白的表达量降低,且Bax蛋白表达量与Bcl-2蛋白表达量的比值增加。In order to investigate the effect of usnic acid on human bladder cancer,cultured human bladder cancer cells T24 and RT4 were treated with different concentrations of usnic acid(12.5,25,50,100,200μmol/L)for 24 h and 48 h,respectively,and the proliferation of T24 and RT4 cells was detected by MTT assay.The re⁃sults showed that intracellular reactive oxygen species(ROS)levels were increased after treatment with usnic acid,and apoptosis of T24 cells was induced through mitochondrial pathway and death receptor pathway:(1)Usnic acid inhibited the proliferation of T24 and RT4 cells in a time-dependent and dose-dependent manner,and the inhibitory rate of usnic acid on T24 cell proliferation was higher.(2)By Hoechst 33342 staining and An⁃nexin V-FITC/PI double staining,apoptotic T24 cells gradually increased with the increase of the concentration of usnic acid.(3)After treatment with usnic acid,the level of intracellular ROS increased,the expression of Bax,cleaved-Caspase-8,cleaved-Caspase-9 and cleaved-Caspase-3 protein increased,the expression of Bcl-2,Caspase-3,Caspase-8 and caspase-9 decreased,and the ratio of Bax protein expression to Bcl-2 pro⁃tein expression increased.

关 键 词：松萝酸 膀胱癌 凋亡 线粒体途径 死亡受体途径 

分 类 号：R285.5[医药卫生—中药学] R737.1[医药卫生—中医学]