黄芪甲苷衍生物HHQ16上调α1酸性糖蛋白改善肝细胞脂质沉积  

Upregulation ofα1-acidglycoprotein to ameliorate hepatocyte lipid accumulation by Astragaloside derivative HHQ16

作　　者：黎翔 代鲜敏 刘霞 孙旸 LI Xiang;DAI Xianmin;LIU Xia;SUN Yang(Department of Clinical Pharmacy,School of Pharmacy,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学药学系临床药学教研室,上海200433

出　　处：《药学实践与服务》2024年第4期141-146,共6页Journal of Pharmaceutical Practice and Service

基　　金：国家自然科学基金项目(82073907,82073842);上海市科委生物医药领域科技支撑项目(20S11902700);上海市2021年度“科技创新行动计划”优秀学术/技术带头人计划项目(21XD1404700)。

摘　　要：目的 研究黄芪甲苷衍生物HHQ 16对肝细胞脂质沉积的作用及机制。方法 在AML12肝细胞上用游离脂肪酸构建脂质沉积模型,检测细胞的甘油三酯和油红染色等反映肝细胞脂质沉积情况。通过实时定量PCR和蛋白免疫印迹法检测α1酸性糖蛋白(ORM)及其上游相关调控因子表达。通过siRNA技术对ORM1的表达进行干扰,确定ORM1是否介导HHQ16的作用。结果 HHQ16显著改善FFA导致的肝脂质沉积,同时HHQ16升高ORM表达,干扰主要亚型ORM1表达后HHQ16改善肝细胞脂质沉积作用被逆转。结论 HHQ16通过升高ORM改善肝细胞脂质沉积。Objective To investigate the effect and mechanism of HHQ 16,a derivative of astragalosideⅣ,on hepatocyte lipid accumulation.Methods Free fatty acids were used to stimulate lipid hepatocyte accumulation.Triglyceride and Oil Red O staining were detected to reflect hepatocyte lipid accumulation.The expression ofα1-acidglycoprotein(ORM)and its regulators were detected by real-time quantitative PCR and immunoblotting.The expression of ORM1 was interfered with siRNA to determine whether it mediated the action of HHQ16.The expression of ORM1 was interfered by siRNA to determine whether it mediated the action of HHQ16.Results HHQ16 significantly ameliorated FFA-induced hepatocyte lipid deposition.HHQ16 elevated ORM expression,and the protective effect of HHQ16 on hepatocyte lipid accumulation was reversed by ORM interference.Conclusion HHQ16 could ameliorate hepatocyte lipid accumulation by elevating ORM.

关 键 词：HHQ16 Α1酸性糖蛋白 肝细胞 脂质沉积 

分 类 号：R285[医药卫生—中药学]