医院制剂蛇药酒抑制眼镜蛇毒酶活性的实验研究  

Experimental study on inhibitory effect of cobra venom enzymes of hospital preparation Sheyao-liquor

作　　者：谭丽华 杨莹莹 庄元烨 张盛花 李耀华 梁平 罗威 黄秋燕 刘思彤 夏星[3] TAN Lihua;YANG Yingying;ZHUANG Yuanye;ZHANG Shenghua;LI Yaohua;LIANG Ping;LUO Wei;HUANG Qiuyan;LIU Sitong;XIA Xing(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning City,Guangxi Zhuang Autonomous Region,530200,China;International Research Center of Biotoxin,Wuzhou Hospital of Traditional Chinese Medicine,Wuzhou City,Guangxi Zhuang Autonomous Region,543003,China;Key Laboratory of TCM Neuro-metabolism and Immunopharmacology of Guangxi Education Department,Guangxi University of Chinese Medicine,Nanning City,Guangxi Zhuang Autonomous Region,530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]梧州市中医医院国际生物毒素研究中心,广西梧州543003 [3]广西中医药大学广西高校中药神经-代谢及免疫药理重点实验室,广西南宁530200

出　　处：《蛇志》2024年第1期1-5,共5页Journal of Snake

基　　金：广西大学生创新创业训练计划项目(项目名称:新医院制剂蛇药酒治疗毒蛇咬伤的药效学研究,项目编号:202210600007);广西壮族自治区中医药管理局(项目名称:广西中药壮瑶药医院制剂质量提升项目,项目编号:GZZJ202029,GZZJ202030,GXZYZ20210230)。

摘　　要：目的探讨医院制剂蛇药酒抗眼镜蛇毒中4种主要酶活性的效果。方法采用不同浓度蛇药酒与蛇毒共孵育作为蛇药酒组,同时设置无蛇药酒的蛇毒对照组。通过琼脂平板法比较蛇药酒组及蛇毒对照组产生透明圈的直径,以检测不同浓度蛇药酒对眼镜蛇毒中磷脂酶A_(2)(PLA_(2))、纤维蛋白原溶酶(以下简称纤溶酶)的抑制作用;分别以福林法和浊度法检测两组的蛋白水解酶和透明质酸酶活性,观察蛇药酒对眼镜蛇毒中蛋白水解酶、透明质酸酶的抑制作用。结果蛇药酒浓度为0.0008、0.004、0.02、0.1、0.5 mg/ml时,对眼镜蛇毒中的PLA_(2)有较强的抑制作用;蛇药酒浓度为1.3、13、130 mg/ml时,可明显抑制纤溶酶活性;蛇药酒浓度为0.5、2、8、32 mg/ml时,对蛇毒中蛋白水解酶抑制作用显著;蛇药酒浓度为0.0625、0.125、0.25、0.5、1 mg/ml时,对眼镜蛇毒中透明质酸酶抑制作用呈剂量依赖性。结论医院制剂蛇药酒在体外有直接抑制眼镜蛇毒中PLA_(2)、纤溶酶、蛋白水解酶及透明质酸酶的作用。Objective To investigate the effect of hospital preparation Sheyao-liquor(Chinese snakebite medicine)on four primary enzyme activities in cobra venom.Methods Using different concentrations of Sheyao-liquor and cobra venom co-incubation as the snake medicinal wine group,while the cobra venom without Sheyao-liquor was used as the snake venom control group,agarose plates were used to compare the inhibitory effects of the Sheyao-liquor group and the snake venom control group on phospholipase A_(2)(PLA_(2))and fibrinolytic enzyme activities;in addition,the protein hydrolase and hyaluronidase activities of the two groups were detected by formalin and turbidimetric methods,respectively,to demonstrate the inhibitory effects of Sheyao on protein hydrolase and hyaluronidase in cobra venom.Results The Sheyao-liquor exhibits a robust inhibitory influence on PLAA_(2) in cobra venom at 0.0008,0.004,0.02,0.1,0.5 mg/ml.Moreover,Sheyao-liquor significantly inhibits fibrinolytic enzyme activity at 1.3,13,130 mg/ml.Furthermore,Sheyao-liquor exhibits a pronounced inhibitory effect on proteolytic enzymes in cobra venom when utilized at 0.5,2,8,32 mg/ml.Importantly,the inhibitory impact of Sheyao-liquor on hyaluronidase in cobra venom displays a dose-dependent relationship at 0.0625,0.125,0.25,0.5,1 mg/ml.Conclusion The hospital preparation Sheyao-liquor could inhibit the activity of phospholipase A_(2),fibrinolytic enzymes,proteolytic enzymes,and hyaluronidase in cobra venom in vitro.

关 键 词：蛇药酒 蛇毒 磷脂酶A_(2) 纤溶酶 蛋白水解酶 透明质酸酶 

分 类 号：R996.3[医药卫生—毒理学]