梨果实石细胞木质素合成调控机制研究进展  

Research progress in the regulation mechanism of lignin synthesis in pear stone cells

作　　者：王红宝 王永博[1] 王晋 李勇[1] 李晓[1] 王迎涛[1] 王亚茹[1] WANG Hongbao;WANG Yongbo;WANG Jin;LI Yong;LI Xiao;WANG Yingtao;WANG Yaru(Shijiazhuang Institute of Fruit Trees,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050061,Hebei,China)

机构地区：[1]河北省农林科学院石家庄果树研究所,石家庄050061

出　　处：《果树学报》2024年第4期750-763,共14页Journal of Fruit Science

基　　金：国家现代农业产业技术体系项目(CARS-28-04);河北省现代种业科技创新专项(21326308D);河北省农林科学院基本科研业务费(2023100102)。

摘　　要：石细胞是由木质素沉积形成的厚壁组织细胞,是制约梨果实品质提升的重要因素。梨果肉石细胞的形成与木质素的生物合成、转移和沉积密切相关。因此,探究梨果实发育过程中木质素合成调控机制对调节石细胞形成及果实品质改良具有重要意义。总结近年来与梨果实木质素合成调控机制有关的研究,对转录因子、激素、糖、钙、活性氧、光质及花粉直感等调控因子在梨果实木质素合成中的作用机制进行综述,旨在为梨果实木质素的调控网络深入研究及品质改良提供参考。Stone cells are sclerenchyma cells formed by deposition of lignin,which is the most signifi-cant factor limiting the quality of pears.Therefore,it is of great significance to explore the mechanism of lignin synthesis regulation in pear fruit development for the genetic regulatory network of stone cell traits.In this paper,the mechanisms of transcriptional factors,hormones,sugars,calcium,reactive oxy-gen species,light quality and pollen sensitivity in lignin synthesis in pear fruits are reviewed,aiming to provide reference for the regulatory network of lignin in pear fruits and genetic improvement of fruit quality.Several transcription factors involved in lignin biosynthesis have been identified in pears,such as MYB,NAC,bZIP,KNOX and zinc finger protein,among which MYB and NAC transcription fac-tors play key regulatory roles in lignin synthesis.Study has showed that PbrMYB169,an R2R3MYB transcription factor of Pyrus bretschneideri,positively regulates lignification of stone cells in pear fruits.On the one hand,PbrMYB24 activates the transcription of lignin and cellulose biosynthesis genes by binding to different cis-element.On the other hand,PbrMYB24 binds directly to the promoters of PbrMYB169 and PbrNSC,activating the gene expression.Moreover,both PbrMYB169 and PbrNSC ac-tivate the promoter of PbrMYB24,enhancing gene expression.Research has identified PbMYB61 and PbMYB308 as candidate transcriptional regulators of stone cell formation,revealing that PbMYB61 reg-ulates stone cell lignin formation by binding to the AC element in the PbLAC1 promoter to upregulate expression.Exogenous application of 200μmol·L-1 NAA can reduce stone cell content and also signifi-cantly decrease the expression level of PbrNSC encoding a transcriptional regulator.In addition,PbrARF13-PbrNSC-PbrMYB132 regulatory cascade mediates the biosynthesis of lignin and cellulose in stone cells of pear fruit in response to auxin signals.Research has found several hormone-responsive el-ements in the upstream regulatory sequences of PbPALs family

关 键 词：梨 石细胞 木质素 调控机制 

分 类 号：S661.2[农业科学—果树学]