加味丹玄口康对口腔黏膜下纤维化中上皮间质转化的调控研究  

Regulation of EMT by Jiawei Danxuan Koukang in Oral Submucous Fibrosis

作　　者：阳艺 周蓉 邓媛文 李婧 朱可可 YANG Yi;ZHOU Rong;DENG Yuanwen;LI Jing;ZHU Keke(Department of Periodontal Mucosa,Changsha Stomatological Hospital,Changsha 410005,China;Department of Stomatology,Changsha Maternal and Child Health Hospital,Changsha 410000,China;Department of Stomatology,the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)

机构地区：[1]长沙市口腔医院牙周黏膜科,湖南长沙410005 [2]长沙市妇幼保健院口腔科,湖南长沙410000 [3]湖南中医药大学第一附属医院口腔科,湖南长沙410007

出　　处：《现代中药研究与实践》2024年第1期30-36,共7页Research and Practice on Chinese Medicines

基　　金：湖南省中医药科研计划项目(2021240)。

摘　　要：目的研究加味丹玄口康对口腔黏膜下纤维化(OSF)中上皮间质转化(EMT)的调控作用及其基于微小RNA(miR)-4725-3p(MIR)/分泌型卷曲相关蛋白4(SFRP4)信号通路的调控机制。方法收集OSF和正常口腔黏膜组织。用槟榔碱刺激人永生化表皮细胞HaCaT模拟OSF细胞模型,并用加味丹玄口康治疗。再用MIR模拟物、MIR抑制剂、MIR阴性对照(NC)分别干预槟榔碱组和加味丹玄口康+槟榔碱组的细胞。分别检测MIR、SFRP4以及纤维化和EMT相关蛋白上皮钙粘蛋白、波形蛋白、细胞角蛋白19的表达,以及MIR和SFRP4的互作关系。结果与正常口腔黏膜组相比,OSF组中MIR表达下调,SFRP4表达上调;MIR对SFRP4起负调控作用。细胞实验中,用槟榔碱构建OSF模型成功;加味丹玄口康处理后MIR、细胞角蛋白19、上皮钙粘蛋白的表达升高,而SFRP4和波形蛋白表达降低;与加味丹玄口康+MIR NC处理组相比,加味丹玄口康+MIR抑制剂组的指标呈现相反的趋势。结论加味丹玄口康可通过调控MIR/SFRP4轴抑制OSF中的上皮间质转化。Objective To investigate the regulatory effect of modified Jiawei Danxuan Koukang on epithelialmesenchymal transition(EMT)in oral submucosal fibrosis(OSF)and its regulatory mechanismal involving the microRNA(miR)-4725-3p(MIR)/secretory curl related protein 4(SFRP4)signaling pathway.Methods OSF tissue samples and normal oral mucosa tissues were collected.Human immortalized epidermal cells(HaCaT)were stimulated with arecoline to simulate OSF model in vitro,and treated with Jiawei Danxuan Koukang.Cells of the arecoline group and the Jiawei Danxuan Koukang+coline group were intervened with MIR mimics,MIR inhibitors,and MIR negative controls(MIR NC)respectively.The expression of MIR,SFRP4,and EMT-and fibrosis-related proteins E-cadherin,Vimentin,and cytokeratin 19 were detected.The interaction between MIR and SFRP4 was detected.Results Compared with normal oral mucosa group,the expression of MIR was downregulated and SFRP4 was up-regulated in submucosal fibrosis group.Also,MIR negatively regulated SFRP4.In cellular experiments,the OSF model was successfully established using arecoline.After treatment with Jiawei Danxuan Koukang,the expressions of MIR,cytokeratin 19 and E-cadherin were increased,while the expressions of SFRP4 and Vimentin were decreased.Compared to the Jiawei Danxuan Koukang+MIR NC treatment group,the Jiawei Danxuan Koukang+MIR inhibitor treatment group exhibited opposite trends in these indicators.Conclusion Jiawei Danxuan Koukang can inhibit epithelial-mesenchymal transition in OSF by regulating MIR/SFRP4 axis.

关 键 词：加味丹玄口康 微小RNA-4725-3p 分泌型卷曲相关蛋白4 口腔黏膜下纤维化 上皮间质转化 

分 类 号：R285.5[医药卫生—中药学]