非干涉无标记三维折射率显微成像的研究进展  

Research Progress on Non-Interferometric Label-Free Three-Dimensional Refractive Index Microscopy

作　　者：仝展 任雪松 张子晗 苗玉彬[1] 孟国香[1] Tong Zhan;Ren Xuesong;Zhang Zihan;Miao Yubin;Meng Guoxiang(School of Mechanical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学机械与动力工程学院,上海200240

出　　处：《激光与光电子学进展》2024年第4期1-28,共28页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(51975361,U1931137)。

摘　　要：光学衍射层析成像以折射率为内源性染剂,非侵入式地获取生物样本的三维结构信息,并有希望实现对活体样本(如活细胞等)的长时间动态观测,在生物医学和生命科学领域具有重要的意义。然而,光学衍射层析成像依赖于弱散射近似和干涉测量,前者极大地限制此项技术在集群细胞和组织等厚样本上的观测表现,后者则会显著地增加成像系统的复杂度。针对上述问题,研究人员开发了一类基于非干涉强度测量原理的衍射层析成像技术。首先,对非干涉强度衍射层析成像进行基本描述,包括其成像系统、成像指标和重建问题等;接下来从非干涉相位恢复、三维光学传递函数、多层递归正向传播模型和神经网络4条技术路线介绍非干涉强度衍射层析成像的研究成果和最新进展,并对上述方法进行对比;最后讨论当前面临的问题和挑战以及未来的研究方向。Optical diffraction tomography(ODT)uses refractive index(RI)as endogenous dyes to non-invasively obtain three-dimensional(3D)structural information of biological samples,and is promising to achieve long-term dynamic observation for living samples(such as live cells,etc.),which is of great significance in the fields of biomedical and life sciences.However,ODT relies on weak scattering approximation and interferometric measurement.The former greatly limits the observation performance of this technology on thick samples such as clustered cells and tissues,and the latter significantly increases the complexity of the imaging system.To address these issues,researchers have developed a class of diffraction tomography based on the principle of non-interferometric intensity measurement.First,a basic description of non-interferometric intensity diffraction tomography(IDT)is provided,including its imaging system,imaging metrics,and reconstruction problem;Then,we introduce the research results and latest progresses of non-interferometric IDT from four technical routes:non-interferometric phase retrieval,3D optical transfer function,multi-layer forward propagation model,and neural network,and compare the above methods;Finally,current problems and challenges as well as future research directions are discussed.

关 键 词：衍射层析成像 非干涉强度测量 正向物理成像模型 三维折射率重建 

分 类 号：O436.1[机械工程—光学工程]