Evaluating the Compatibility Mechanism of Shengxian Decoction Based on an Excretion Study of 18 Bioactive Constituents in Rat Biosamples  

作　　者：Tao Pang Nan Weng Yong Chen Cui-Yun Huang Mei Sun Feng Zhang Wan-Sheng Chen 

机构地区：[1]Department of Pharmacy,Changzheng Hospital,Naval Medical University(Second Military Medical University),Shanghai,China [2]Department of Pharmacognosy Shenyang Pharmaceutical University,Shenyang,Liaoning Province,China [3]Traditional Chinese Medicine Resource and Technology Center,Shanghai University of Traditional Chinese Medicine,Shanghai,China

出　　处：《World Journal of Traditional Chinese Medicine》2024年第1期40-52,共13页世界中医药杂志（英文）

基　　金：supported by National Key R&D Program of China(2022YFC3501700);National Natural Science Foundation of China(82274059);Science and Technology Commission of Shanghai Municipality(22S21901900);Organizational Key Research and Development Program of Shanghai University of Traditional Chinese Medicine(2023YZZ02)。

摘　　要：Introduction:The Shengxian decoction(SXT),a Chinese herbal medication used to treat heart failure,is composed of Astragali Radix,Anemarrhenae Rhizoma,Bupleuri Radix,Cimicifuage Rhizoma,and Platycodonis Radix.Knowledge of the excretion of the active compounds in this herbal medication is vital for investigating the underlying mechanisms behind its compatibility.However,this remains unclear.Methods:Liquid chromatography coupled with mass spectrometry was performed in both positive and negative modes to assay 18 constituents of SXT from rat urinary,fecal,and biliary samples.Results:The methodology was validated and showed good linearity(r≥0.9),precision,stability,repeatability,and recovery.The relative standard deviations and relative errors for the precision and accuracy did not exceed 15%.The recoveries of all the compounds ranged from 77.37%to 114.82%,and the matrix effect was between 82.53%and 105.71%.The results suggested that,when combined with Platycodonis Radix,the levels of 14 constituents from the four herbs were reduced in the urine,while the levels of six constituents were reduced in the feces.Conclusions:The comparative excretion results indicated that Platycodonis Radix reduced the excretion of compounds in SXT,demonstrating the compatibility mechanism and holistic properties of these compounds,favoring the pharmacological effects of SXT.

关 键 词：Compatibility EXCRETION liquid chromatography coupled with mass spectrometry Platycodonis Radix Shengxian decoction 

分 类 号：R285.5[医药卫生—中药学]