CRISPR/Cas12a技术在动物疫病检测方面的应用  

Application of CRISPR/Cas12a Technology in Animal Disease Detection

作　　者：孔玉方 王慧煜[1] 袁向芬[1] 许晓琳 吴绍强[1] KONG Yufang;WANG Huiyu;YUAN Xiangfen;XU Xiaolin;WU Shaoqiang(Institute of Animal Inspection and Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院动物检验与检疫研究所,北京大兴100176

出　　处：《中国兽医杂志》2024年第4期92-97,共6页Chinese Journal of Veterinary Medicine

基　　金：海南省重点研发项目(ZDYF2023XDNY061)。

摘　　要：CRISPR/Cas系统是由簇状规则间隔的短回文重复序列(CRISPR)和CRISPR相关蛋白(Cas)组成的一种基因编辑技术,可以特异地切割和识别靶标核酸基因。该技术具有检测速度快、灵敏度高和特异性强的优势,因此被广泛应用于动物疫病检测领域。本文就CRISPR/Cas12a结合等温核酸扩增技术和可视化技术在动物疫病检测中的应用进行综述,并对其应用前景进行展望,以期为我国的养殖业和进境动物疫病的监测提供技术支撑。The CRISPR/Cas system is a gene editing technology composed of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas),which can specifically cleave and identify target nucleic acid genes.This technology has the advantages of fast detection speed,high sensitivity,and strong specificity,and is therefore widely used in the field of animal disease detection.This article reviews the application of CRISPR/Cas12a combined with isothermal nucleic acid amplification technology and visualization technology in animal disease detection,and prospects its application,aiming to provide technical support for the monitoring of animal diseases in China's breeding industry and imported animals.

关 键 词：簇状规则间隔的短回文重复序列及其相关蛋白12a(CRISPR/Cas12a) 动物疫病 核酸检测 试纸条 

分 类 号：S858.28[农业科学—临床兽医学]