辐射合成的温敏材料上兔角膜基质细胞的培养  

Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization

作　　者：王伟[1] 张鹏飞 陈春霞[1] 钱莉[1] 陈建苏[3] Wang Wei;Zhang Pengfei;Chen Chunxia;Qian Li;Chen Jiansu(Department of Ophthalmology,Tongling People s Hospital,Tongling 244000,Anhui Province,China;The First Affiliated Hospital of Wannan Medical College,Yijishan Hospital,Wuhu 241000,Anhui Province,China;Institute of Ophthalmology,School of Medicine,Jinan University,Guangzhou 510632,Guangdong Province,China)

机构地区：[1]中国安徽省铜陵市人民医院眼科,244000 [2]皖南医学院第一附属医院弋矶山医院,安徽省芜湖市241000 [3]暨南大学基础医学与公共卫生学院眼科研究所,广东省广州市510632

出　　处：《国际眼科杂志》2024年第5期686-690,共5页International Eye Science

基　　金：安徽省自然科学基金资助(No.1808085MH253);2023年蚌埠医学院自然重大项目(No.2023byzd034)。

摘　　要：目的:探索以电子加速器合成具有温度敏感特性的聚异丙基丙烯酰胺凝胶(PNIPAAm)及接枝有PNIPAAm水凝胶的培养皿的方法,及兔角膜基质细胞在温敏材料PNIPAAm水凝胶上的生长条件及特性,以及利用PNIPAAm水凝胶获得的细胞片。方法:55%N-异丙基丙烯酰胺(NIPAAm)单体和0.5%的N,N’-亚甲基双丙烯酰胺的异丙醇溶液70μL,加入到35 mm培养皿上,电子加速器下辐射合成温敏材料(PNIPAAm),后续处理后,接种兔角膜基质细胞体外培养。结果:按本实验中的单体配方以及辐射合成方案,能够在培养皿表面合成PNIPAAm,角膜基质细胞能在部分接枝了PNIPAAm的培养皿上生长,并能成片脱离。结论:使用辐射合成温敏培养皿能够获得单层及多层无载体的角膜基质细胞片。AIM:To explore the synthesis of thermo-sensitive poly N-isopropylacry-lamide(PNIPAAm)and the petri dish grafted with PNIPAAm hydrogels by the electron accelerator,as well as the growth conditions and the biological characteristics of rabbit corneal stromal cells on thermo-sensitive PNIPAAm hydrogels,and the cell sheets obtained from the PNIPAAm hydrogels.METHODS:NIPAAm monomer was dissolved in 2-propanol at concentrations of 55%with 0.5%N,N’-Methylenebisacry-lamide(MBA).Solution(70μL)was added and spread uniformly over 35 mm petri dish.These dishes were immediately subjected to irradiation.After follow-up treatment,rabbit corneal stromal cells were cultured on thermo-sensitive petri dish in vitro.RESULTS:According to the monomer formula and radiation synthesis scheme in this experiment,PNIPAAm can be synthesized on the surface of the petri dish.Rabbit corneal stromal cells grew well in the thermo-sensitive surface and can be separated into sheets.CONCLUSION:The single and multilayer carrier-free cell sheets can be obtained from the use of thermo-sensitive petri dish.

关 键 词：聚异丙基丙烯酰胺凝胶(PNIPAAm) 温敏材料 细胞片 角膜基质 细胞培养 

分 类 号：R318.08[医药卫生—生物医学工程]