中药曲苓化浊方的指纹图谱研究和提取工艺优化  

Study on fingerprint and optimization of extraction process of Qulinghuazhuo prescription

作　　者：何芸岸 刘秀秀[1] 廖显军 刘梅 李雯皎 付春梅[1] HE Yunan;LIU Xiuxiu;LIAO Xianjun;LIU Mei;LI Wenjiao;FU Chunmeli(West China School of Pharmacy,Sichuan University,Chengdu,Sichuan,610041 P.R.China;Xuanhan Chinese Medicinal Hospital,Xuanhan,Sichuan,636150P.R.China)

机构地区：[1]四川大学华西药学院,四川成都610041 [2]宣汉县中医院,四川宣汉636150

出　　处：《华西药学杂志》2024年第2期174-178,共5页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81803705);四川大学-达州市人民政府校市战略合作专项资金(2021CDDZ-20)。

摘　　要：目的建立曲苓化浊方的HPLC指纹图谱,优化其提取工艺。方法采用Sapphire C18色谱柱(250 mm×4.6 mm,5μm),以0.1%磷酸水为流动相A、乙腈为流动相B,梯度洗脱,流速1.0 mL·min^(-1),柱温45℃,在240 nm处进行检测,获得曲苓化浊方的指纹图谱和相似度结果。设计三因素三水平正交试验,考察提取溶剂、提取时间和料液比对主要成分含量的影响。结果建立了曲苓化浊方的指纹图谱,指认了6个重要特征峰,相似度评价表明:10批曲苓化浊方的质量有一定差异。最佳提取工艺为:以甲醇作提取溶剂,料液比1:6,超声提取90 min。结论成功建立了曲苓化浊方的指纹图谱,优化了提取工艺,为方剂的药效物质基础和质量控制研究奠定了基础。OBJECTIVE To establish an HPLC fingerprint of Qulinghuazhuo prescription and to optimize its extraction process.METHODS A Sapphire Cis column(250 mm×4.6 mm,5μm)was used with mobile phase of 0.1%phosphoric acid water(A)and acetonitrile(B)for gradient elution at a flow rate of 1.O mL·min^(-1)under the column temperature of 45℃,and the detection wavelength was 240 nm.Orthogonal experiments were designed to investigate the effects of extraction solvent,extraction time and solid-liquid ratio on the influence of the content of main components.RESULTS SThe fingerprint of Qulinghuazhuo prescription was established and six important chromatographic peaks were identified.The results of similarity evaluation showed that the quality of ten batches of Qulinghuazhuo prescription had some differences.The optimal extraction process was ultrasonic extracting Qulinghuazhuo prescription with 100%methanol at the ratio of 1 g:6 mL for 90 min.CONCLUSION The fingerprint of Qulinghuazhuo prescription was successfully established and the extraction process was optimized,which lays the foundation for the research on the material basis of medicinal effect and quality control of the prescription.

关 键 词：高效液相色谱法 高脂血症 曲苓化浊方 指纹图谱 提取工艺 正交试验 聚类分析 相似度评价 

分 类 号：R917[医药卫生—药物分析学]