孤雌卤虫雌激素相关受体基因(ERR)的表达特性分析  

Analysis of Expression Characteristics of Estrogen-Related Receptor Gene(ERR)in Artemia parthenogenetica

作　　者：刘雪 韩学凯 欧阳雪梅 隋丽英 LIU Xue;HAN Xuekai;OUYANG Xuemei;SUI Liying(College of Marine and Environmental Sciences,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学海洋与环境学院,天津300457

出　　处：《天津科技大学学报》2024年第2期34-41,共8页Journal of Tianjin University of Science & Technology

基　　金：天津市自然科学基金项目(18JCQNJC78500);教育部长江学者和创新团队发展计划资助项目(IRT-17R81)。

摘　　要：雌激素通过其相关受体进而影响动物的生殖发育与成熟。为了探究雌激素相关受体基因ERR在孤雌卤虫卵巢发育和成熟过程中发挥的作用,本研究对孤雌卤虫(Artemia parthenogenetica)的ERR基因进行了克隆及相关生物信息学分析,并对该基因在不同组织(卵巢、头部、躯干)和卵巢在不同发育时期〔卵黄发生早期(early oocytes,EO)、卵黄发生晚期(late oocytes,LO)、早期胚胎(early embryos,EE)、晚期胚胎(later embryos,LE)〕的表达特征进行了探究。结果显示,ERR基因的开放阅读框(ORF)长1536 bp,共编码521个氨基酸,编码蛋白质的相对分子质量和等电点分别为5.7114×10^(4)和5.06,该蛋白质具有两个结构域,没有信号肽和跨膜结构。蛋白质的二级结构主要以无规则卷曲(46.97%)和α-螺旋(40.7%)为主,三级结构与二级结构的结果一致。在NJ系统进化树中,孤雌卤虫(A.parthenogenetica)与大型溞(Daphnia magna)和苏拉威西秀体溞(Diaphanosoma celebensis)最为相近,而与湿木白蚁(Zootermopsis nevadensis)、台湾乳白蚁(Coptotermes formosanus)的亲缘关系较远。表达特征结果显示,ERR基因在孤雌卤虫头部组织中的表达量显著高于卵巢组织和躯干组织中的表达量,且随着卤虫卵巢的发育,表达量逐渐上升,在EE达到最高,随后在LE显著下降(P<0.01)。Estrogen affects the development and maturation of reproductive system of animals through their interactions with estrogen-related receptors.In order to explore the role of estrogen-related receptor gene ERR in ovarian development and maturation of Artemia parthenogenetica,the ERR gene was cloned and analyze by bioinformatics.Moreover,the expression characteristics of this gene in different tissues(ovaries,head and trunk)and gonads at different developmental stages of A.parthenogenetica(early oocytes(EO),late oocytes(LO),early embryos(EE)and later embryos(LE))were further studied.The results showed that the ORF length of ERR gene was 1536 bp,encoding 521 amino acids,and the relative molecular weight and isoelectric point of encoded protein were 5.7114×10^(4) and 5.06,respectively.The protein had two domains with no signal peptide and transmembrane structure.Irregular volumes(46.97%)and α-helix(40.7%)dominated the secondary structure and the tertiary structure corresponded to the secondary structure.In the NJ phylogenetic tree,A.parthenogenetica was found to be most closely related to Daphnia magna and Diaphanosoma celebensis,but was distant from Zootermopsis nevadensis and Coptotermes formosanus.Analysis of the expression characteristic showed that the expression level of ERR gene in head tissues was significantly higher than that in ovarian and trunk and with the maturation of A.parthenogenetica ovary,the expression level of ERR gene was up-regulated gradually,reaching the highest level in EE,and then significantly down-regulated in LE(P<0.01).

关 键 词：孤雌卤虫 雌激素相关受体 生物信息学特征 基因表达 卵巢发育 

分 类 号：Q74[生物学—分子生物学]