盐胁迫下Spd对非洲凤仙生理指标及总黄酮含量的影响  

The effect of Spd on physiological indexes and total flavonoids content of Impatiens wallerana Hook.f.under NaCl stress

作　　者：何会流[1] HE Huiliu(Chongqing City Management College,Chongqing 401331,China)

机构地区：[1]重庆城市管理职业学院,重庆401331

出　　处：《内江师范学院学报》2024年第4期81-86,共6页Journal of Neijiang Normal University

基　　金：重庆城市管理职业学院校级科研项目(2022NDXM06)。

摘　　要：以花期非洲凤仙(Impatiens wallerana Hook.f.)为试验试材,研究外源亚精胺(Spd)对100 mmol/L NaCl胁迫下非洲凤仙生理特性以及总黄酮含量的影响.设置不同浓度的Spd处理组:0 mmol/L、0.2 mmol/L、0.4 mmol/L、0.6 mmol/L、0.8 mmol/L,测定处理时间12 d下非洲凤仙各项生理指标及总黄酮含量.盐胁迫下,非洲凤仙中可溶性糖、可溶性蛋白降低,抗氧化酶(SOD、POD、CAT)活性降低,脯氨酸含量、丙二醛(MDA)含量升高,膜脂过氧化程度加剧;经Spd处理后,可溶性糖、可溶性蛋白和脯氨酸含量均升高,抗氧化酶活性呈上升趋势,丙二醛含量降低,总黄酮含量升高.研究表明,外源Spd可以缓解盐胁迫对非洲凤仙的伤害,提升总黄酮含量且以0.6 mmol/L Spd处理效果最佳.Effects of exogenous spermidine(Spd)on the physiological characteristics and total flavonoid of Impatiens wallerana Hook.f.under the NaCl(100mmol/L)stress condition were studied.The Impatiens wallerana Hook.f.were cultivated in 0,0.2,0.4,0.6,0.8 mmol/L Spd for 12 days and then the determination of their physiological characteristics and total flavonoid content is carried out.The results showed that the soluble sugar,soluble protein and the activities of SOD,POD,CAT decreased,the content of proline and malondialdehyde(MDA)increased,and the membrane lipid peroxidation intensified under NaCl stress.After the Spd treatment,the content of soluble sugar,soluble protein and proline have all increased,and the activities of SOD,POD,CAT take on an increasing trend;the malondialdehyde content decreased,and the total flavonoids content increased.As a result,Spd could alleviate the damage inflicted by the NaCl stress upon Impatiens wallerana Hook.f.,and increase the total flavonoid content,with the best treatment effect observed when treated with 0.6mmol/L Spd.

关 键 词：非洲凤仙 盐胁迫 外源Spd 生理特性 总黄酮 

分 类 号：S681.1[农业科学—观赏园艺]