卵巢浆液性癌中miR-182和E-cadherin的表达及意义  

Expression and significance of miR-182 and E-cadherin in ovarian serous carcinoma

作　　者：黄凡[1] 彭肖龙 贺聪[1] Huang Fan;Peng Xiaolong;He Cong(Oncology Department II,Zhongxiang People's Hospital,Jingmen,Hubei,429001,China)

机构地区：[1]钟祥市人民医院肿瘤内科II,湖北荆门429001

出　　处：《青岛医药卫生》2024年第2期101-104,共4页Qingdao Medical Journal

摘　　要：目的检测卵巢浆液性癌中微小RNA-182(microRNA-182,miR-182)和上皮型粘附素(E-cadherin)的表达,探讨其相关性及临床意义。方法选择行卵巢浆液性癌根治性手术的患者59例作为研究组,选择行手术治疗的卵巢浆液性囊腺瘤患者59例作为对照组,应用实时荧光定量PCR法检测组织中miR-182的表达,应用免疫组化法检测组织E-cadherin的表达。结果研究组中miR-182的表达量(1.83±0.32)明显高于对照组(1.31±0.31)(t=4.59,P=0.012),研究组中E-cadherin表达阳性率(47.46%)明显低于对照组(84.75%)(X~2=18.31,P=0.000)。miR-182和E-cadherin在卵巢浆液性癌不同级别(2.01±0.29 vs.1.66±0.22)(27.59%vs.66.67%)、不同肿瘤最大径(1.93±0.22 vs.1.64±0.39)(28.21%vs.85.00%)及有无脉管癌栓(1.80±0.15 vs.2.03±0.20)(20.00%vs.53.06%)的比较中差异有统计学意义(P<0.05),而在不同年龄、有无淋巴结转移分组的比较中差别无统计学意义(P>0.05)。线性相关分析显示研究组中miR-182与E-cadherin呈负相关性(r=-0.57,P=0.008)。生存分析显示miR-182的表达与患者生存时间有关(P<0.05)。结论miR-182和E-cadherin在卵巢浆液性癌中异常表达且具有相关性,组织中miR-182高表达提示不良预后。Objective To detect the expression of microRNA-182(miR-182)and E-cadherin in o-varian serous carcinoma and explore their correlation and clinical significance.Methods 59 pa-tients who underwent radical surgery for ovarian serous carcinoma were selected as the research group,and 59 patients with ovarian serous cystadenoma were selected as the control group.Quantitative real-time fluorescence PCR was used to detect the expression of miR-182,immuno-histochemistry was used to detect the expression of E-cadherin in tissues.Results The expres-sion of miR-182was significantly higher in the study group(1.83±0.32)than that in the control group(1.31±0.31)(t=4.59,P=0.012).The positive rate of E-cadherin expression was signifi-cantly lower in the study group(47.46%)than that in the control group(84.75%)(X2=18.31,P=0.000).MiR-182 and E-cadherin are associated with different grades of ovarian serous carci-noma(2.01±0.29 vs.1.66±0.22)(27.59%vs.66.67%),and different maximum tumor diam-eters(1.93±0.22 vs.1.64±0.39)(28.21%vs.85.00%)and vascular tumor thrombus(1.80±0.15 vs.2.03±0.20)(20.00%vs.53.06%),the difference was statistically significant(P<0.05).Linear correlation analysis showed a negative correlation between miR-182 and E-cadherin in the study group(r=-0.57,P=0.008).Survival analysis showed that the expression of miR-182 was related to survival time(P<0.05).Conclusions miR-182 and E-cadherin are abnormally ex-pressed in ovarian serous carcinoma.High expression of miR-182 of tissues indicates poor prognosis.

关 键 词：卵巢浆液性癌 微小RNA-182 E-CADHERIN 临床病理特征 预后 

分 类 号：R271.1[医药卫生—中医妇科学]