柞蚕蛹培养蝉花的孢子粉和孢梗束的代谢组分析  

METABOLOMICS DIFFERENCES BETWEEN THE SPORES AND COREMIA OF ISARIA CICADAE CULTURED ON ANTHERAEA PERNYI

作　　者：何亚琼 彭凡 赵铖 黄勃[2] 胡丰林[2] HE Yaqiong;PENG Fan;ZHAO Cheng;HUANG Bo;HU Fenglin(Public Foundation Department,North Anhui Health Vocational College,Suzhou,Anhui 234000,China;College of Life Sciences,Anhui Agricultural University,Hefei,Anhui 230036,China)

机构地区：[1]皖北卫生职业学院公共基础部,安徽宿州234000 [2]安徽农业大学生命科学院,安徽合肥230036

出　　处：《井冈山大学学报（自然科学版）》2024年第2期65-72,共8页Journal of Jinggangshan University (Natural Science)

基　　金：安徽省教育厅自然科学重点项目(KJ2021A1566)。

摘　　要：已有研究表明,人工培养蝉花与野生蝉花的功效非常相似,具有替代野生蝉花的潜力。本实验通过基于HPLC-TOF/MS的代谢组学的方法,将人工蝉花孢子部分和孢梗束部分进行代谢物组差异分析比较。分析柞蚕蛹培养的蝉花孢子部分和孢梗束部分代谢产物的差异,将为开发蝉花活性部位产品提供理论依据。结果表明,柞蚕蛹培养蝉花的孢子部分和孢梗束部分之间的代谢产物差异显著。经谱库检索鉴定,得到88个差异化合物,其中78个化合物在孢子部分含量较高。柞蚕蛹培养蝉花的孢子和孢梗束之间代谢组显著不同,孢子部分含有较多的生物活性物质和营养物质,因此,柞蚕蛹培养蝉花的孢子粉具有较大开发潜力。Researchs showed that the wild and cultured I.cicadae have similar efficacy,which shows the replace potential of the cultured one.The metabolic difference between the spores and coremia was compared by HPLCTOF/MS-based metabolomics to reveal their difference.This research will provide a theoretical basis for development of the active part of the cultured I.cicadae.The results showed that there are significant metabolic differences between the spores and coremia of cultured I.cicadae including 88 differential metabolites obtained by database queries and analysis.Among them,78 compounds have higher contents in the spores.The spores and coremia of cultured I.cicadae showed significant different metabolomics,and the spores contained relatively higher level of bioactive compounds and nutrients,suggesting the more development potential of spores of cultured I.cicadae.

关 键 词：蝉花虫草 代谢组差异 HPLC-TOF/MS 

分 类 号：S567.3[农业科学—中草药栽培]