软骨及姜黄联合葛根薏仁提取物对膝骨关节炎大鼠软骨损伤的改善作用  

Improvement Effects of Co-administration of Cartilage Extract,Turmeric Extract,Pueraria Lobata and Coix Seed Extract on Cartilage Damage of Rats with Knee Osteoarthritis

作　　者：丁刘刚 关婷 缪金典 方兰婷 侯少贞[3] 苏国万[1] 周勇 DING Liugang;GUAN Ting;MIAO Jindian;FANG Lanting;HOU Shaozhen;SU Guowan;ZHOU Yong(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,Guangdong,China;Infinitus(China)Company Ltd.,Guangzhou 510665,Guangdong,China;School of Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]无限极(中国)有限公司,广东广州510665 [3]广州中医药大学中药学院,广东广州510006

出　　处：《华南理工大学学报（自然科学版）》2024年第4期17-25,共9页Journal of South China University of Technology(Natural Science Edition)

基　　金：广东省重点领域研发计划项目(2020B020226005);华南理工大学中央高校基本科研业务费专项资金资助项目(2022ZYGXZR0074)。

摘　　要：系统研究了姜黄提取物、软骨提取物、葛根薏仁提取物单独及联合使用对膝骨关节炎大鼠软骨损伤的改善作用,主要从减少膝关节肿胀(炎症)、促进软骨细胞修复和关节基质修复方面,研究了3种提取物在大鼠膝骨关节炎中的协同效应。结果显示:姜黄提取物在抑制关节肿胀、改善软骨损伤方面有较明显的作用,具体表现在能较好地改善Ⅱ型胶原蛋白(CollagenⅡ)、基质金属蛋白酶3(MMP3)、软骨寡聚基质蛋白(COMP)、肿瘤坏死因子-α(TNF-α)及超氧化物歧化酶(SOD)的水平;软骨提取物主要通过改善MMP3、COMP、TNF-α、前列腺素E2(PGE2)及SOD水平起到明显改善关节炎病变的效果;葛根薏仁提取物能够提高CollagenⅡ及SOD水平,抑制MMP3、COMP、TNF-α和白细胞介素1β(IL-1β)的升高,从而改善大鼠膝关节病变程度;3种提取物的复合使用对大鼠膝关节软骨的修复作用最为突出,且对各生化指标的异常改变均有更佳的改善效果。由此可见,通过对姜黄提取物、软骨提取物及葛根薏仁提取物的比例优化,可开发出具有显著抗炎和修复软骨作用的功能性食品,为实现“健康中国2030”添砖加瓦。This paper studied the synergistic effects of turmeric extract,cartilage extract,Pueraria lobata and Coix(Coix lacryma-jobi)seed extract alone and the combination on the improvement of cartilage damage of rats with knee osteoarthritis.The synergistic effects of the three extracts in rat knee osteoarthritis were evaluated mainly by reducing knee swelling(inflammation),protecting chondrocyte and repairing joint cartilage.The results show that the turmeric extract has a more obvious effect in inhibiting joint swelling and improving cartilage damage,which is specifically manifested in the ability to better improve the levels of CollagenⅡ,matrix metalloproteinase 3(MMP3),cartilage oligomeric matrix protein(COMP),tumor necrosis factor-alpha(TNF-α)and superoxide dismutase(SOD).The cartilage extract significantly improves the arthritic lesions by improving the levels of MMP3,COMP,TNF-α,prostaglandin E2(PGE2)and SOD.Pueraria lobata and Coix seed extract improves the levels of CollagenⅡand SOD,inhibits the elevation of MMP3,COMP,TNF-αand interleukin 1β(IL-1β),and improves cartilage damage of rats with knee osteoarthritis.The combined use of the three extracts exhibits better effects on the repair of rat knee cartilage.These data imply that by optimizing the ratio of turmeric extract,cartilage extract and Pueraria lobata and Coix seed extract,functional foods can be developed with significant anti-inflammatory and cartilage repairing effects and contribute to the realization of“Healthy China 2030”.

关 键 词：软骨提取物 姜黄提取物 葛根薏仁提取物 膝骨关节炎 软骨修复 

分 类 号：R684.3[医药卫生—骨科学]