基于贻贝仿生构建高效抗菌及良好血液相容性的聚胺-酚表面  

Mussel Mimicking Polyamine-Phenol Surface with Efficient Antibacterial Properties and Good Hemocompatibility

作　　者：孟昊天 李鹏程 魏嘉佳 王文轩 李天瑀 黄楠[1] 涂秋芬[1,2] 熊开琴[2] 杨志禄 MENG Haotian;LI Pengcheng;WEI Jiajia;WANG Wenxuan;LI Tianyu;HUANG Nan;TU Qiufen;XIONG Kaiqin;YANG Zhilu(Key Laboratory of Advanced Technology of Materials of Education Ministry,School of Materials Scienceand Engineering,College of Medicine,Southwest Jiaotong University,Chengdu 610031,China;Institute of Biomedical Engineering,College of Medicine,Southwest Jiaotong University,Chengdu 610031,China;Dongguan People's Hospital,Affiliated Dongguan Hospital,Southern Medical University,Guangdong Dongguan 523059,China)

机构地区：[1]西南交通大学材料科学与工程学院材料先进技术教育部重点实验室,成都610031 [2]西南交通大学医学院生物医学工程研究院,成都610031 [3]南方医科大学附属东莞医院东莞市人民医院,广东东莞523059

出　　处：《表面技术》2024年第8期173-183,共11页Surface Technology

摘　　要：目的赋予血液接触类材料优异的表面抗菌性能。方法受天然贻贝黏附现象的启发,通过多巴胺(Dopamine,DA)、ε-聚赖氨酸(ε-Poly-L-lysine,ε-PL)、高碘酸钠(Sodium Periodate,NaIO4)构建一种简易、快速的聚胺-酚涂层(ε-PL@PDA),同时利用ε-PL丰富的氨基质子化形成的阳离子,实现表面高效抗菌的目的。其中DA和ε-PL通过共价交联形成酚胺聚合物,酚胺共同介导基底材料黏附形成涂层。通过傅里叶变换红外光谱(FTIR)、X射线光电子能谱(XPS)、场发射扫描电镜(SEM)、椭圆偏光、氨基定量、水接触角(WCA)等材料学表征评价ε-PL@PDA涂层的理化性能;通过菌落计数法、细菌活/死染、液体法等细菌实验评价涂层抗菌性能;通过内皮细胞黏附与增殖实验评价其细胞相容性;通过血小板黏附与激活及半体内血液循环实验验证其血液相容性。结果材料学表征结果证明,ε-PL@PDA富氨基涂层成功制备,同时ε-PL的浓度大小会影响涂层的沉积过程;细菌实验表明,ε-PL@PDA涂层具备优异的抗菌性能,当ε-PL质量浓度为3 mg/mL时,对大肠杆菌和表皮葡萄球菌的抑制率高达91%;细胞实验和血液相容性实验表明,相较于316不锈钢(316L SS),ε-PL的质量浓度为3、30 mg/mL时,涂层均不会促进细胞毒性增强、血小板黏附和激活以及血栓形成。结论ε-PL@PDA涂层能够在不影响细胞相容性和血液相容性的基础上有效提高表面抗菌能力,因此,该高效便捷且适用性强的表面抗菌策略或有助于解决临床血液接触类器械细菌感染等问题。In recent years,blood contact devices have brought great convenience to clinical treatments,but they are severely restricted due to bacterial-related infections.Bacteria-related infections not only lead to partial dysfunction of the devices,but also cause a series of complications such as bacteremia and inflammations,which may threaten the patient's life.For purpose of enhancing the antibacterial properties of the blood-contact materials,a simple and rapid polyamine-phenol coating(ε-PL@PDA)was constructed by dopamine(Dopamine,DA),ε-Poly-L-lysine(ε-PL),and sodium periodate(Sodium period,NaIO4)on the basis of the natural mussel adhesion phenomenon and mechanism in this study.At the same time,theε-PL@PDA coating utilized the cations formed by the protonation of abundant amino groups ofε-PL to realize the purpose of high-efficiency antibacterial properties.DA andε-PL formed phenolic amine polymers through covalent crosslinking,and then jointly mediated the adhesion of substrate materials to form the coating.Firstly,the base material(316L SS)was soaked in ammonia water,hydrogen peroxide,and hot water for 1 hour according to the volume ratio of 1∶1∶3,and then washed by RO,UP,and absolute ethyl alcohol respectively.DA(3 mg/mL),NaIO4(3 mg/mL)were prepared with UP,andε-PL was configured in proportions of 0.3,3,and 30 mg/mL.They were mixed uniformly according to the volume ratio of 1∶1∶1,then immersed in the surface of the 316L SS,and reacted for 3 hours at room temperature to obtain theε-PL@PDA coating.According to the different feeding ratios ofε-PL,they were named asε-PL/0.3@PDA,ε-PL/3@PDA,ε-PL/30@PDA coatings respectively.The physical and chemical properties ofε-PL@PDA coatings were evaluated by material characterizations such as FTIR,XPS,SEM,ellipsometry,amino group quantification,and WCA.The antibacterial properties were evaluated by the colony counting method,liquid method and fluorescent staining of live/dead bacteria.The adhesion and proliferation of endothelial cells were used for evaluating

关 键 词：贻贝仿生 多巴胺(DA) ε-聚赖氨酸(ε-PL) 表面改性 抗菌 

分 类 号：TB34[一般工业技术—材料科学与工程]