探讨恒温扩增法在下呼吸道感染病原体检测的价值  

Exploration of the Value of Constant Temperature Amplification Method in Detecting Pathogens in Lower Respiratory Tract Infections

作　　者：杨增利 陈洁[3] 惠开元[4] 顾洁 蒋晓东[4] YANG Zengli;CHEN Jie;HUI Kaiyuan;GU Jie;JIANG Xiaodong(Medical College of Yangzhou University,Yangzhou 225000,China;Department of infectious diseases,The first people's Hospital of Guannan County,Lianyungang 222500,China;Department of gastroenterology,Subei people's Hospital,Yangzhou 225000,China;Precision Medical Laboratory,The first people's Hospital of Lianyungang City,Lianyungang 222000,China)

机构地区：[1]扬州大学医学院,江苏扬州225000 [2]灌南县第一人民医院感染性疾病科,江苏连云港222500 [3]苏北人民医院消化科,江苏扬州225000 [4]连云港市第一人民医院精准医学实验室,江苏连云港222000

出　　处：《工业微生物》2024年第2期148-151,共4页Industrial Microbiology

摘　　要：探究运用恒温扩增法(Loop—mediated isothermal amplification,LAMP)在感染人群病原体诊断方面的价值。收集2022年1-8月255名下呼吸道感染患者的痰液或肺泡灌洗液(Bronchoalveolar lavage fluid,BALF),运用LAMP法和痰培养法同时对其进行检测,分析病原体检出情况、恒温扩增芯片法与痰培养法符合率。运用LAMP法检出阳性标本200例,总阳性率为78.43%,共检出呼吸道病原体10种;运用痰培养法检测出阳性标本80例,总阳性率为31.37%,共检出呼吸道病原体8种。两种方法检出的铜绿假单胞菌的阳性率具有高度一致性(Kappa=0.516),鲍曼不动杆菌、肺炎克雷伯菌的阳性率具有中等一致性(0.4<Kappa<0.6)。恒温扩增法的病原体检出率显著高于传统痰培养法;相较于金标准痰培养法,其对苛养菌的检测具有较好的一致性。但LAMP法检测时间更短、总阳性率更高,更有助于临床医生快速精准地识别所感染的病原体。The paper explores the value of using Loop mediated isothermal amplification(LAMP)in diagnosing pathogens in infected populations.By collecting sputum or bronchoalveolar lavage fluid(BALF)from 255 patients with lower respiratory tract infections from January 2022 to August 2022,this paper uses LAMP method and sputum culture method to detect them simultaneously,and analyzes the detection of pathogens,the consistency rate of constant temperature amplification chip method and sputum culture method.The paper detected 200 positive specimens using LAMP method,with a total positive rate of 78.43%,and a total of 10 respiratory pathogens were detected;80 positive specimens were detected using sputum culture method,with a total positive rate of 31.37%,and a total of 8 respiratory pathogens were detected.The positive rates of Pseudomonas aeruginosa detected by the two methods are highly consistent(Kappa=0.516);The positive rates of Acinetobacter baumannii and Klebsiella pneumoniae showed moderate consistency(0.4<Kappa<0.6).The pathogen detection rate of constant temperature amplification method is significantly higher than that of traditional sputum culture method;Compared with the gold standard sputum culture method,the detection of highly pathogenic bacteria has good consistency.But the LAMP method has a shorter detection time and a higher overall positive rate,which is more helpful for clinical doctors to quickly and accurately identify the pathogens of infection.

关 键 词：下呼吸道感染 恒温扩增法 痰培养 病原体 

分 类 号：R446.5[医药卫生—诊断学]