Plant RNA-binding proteins:Phase separation dynamics and functional mechanisms underlying plant development and stress responses  

作　　者：Sheng Fan Yu Zhang Shaobo Zhu Lisha Shen 

机构地区：[1]Temasek Life Sciences Laboratory,1 Research Link,National University of Singapore,1 Research Link,Singapore 117604,Singapore [2]Department of Biological Sciences,National University of Singapore,14 Science Drive 4,Singapore 117543,Singapore

出　　处：《Molecular Plant》2024年第4期531-551,共21页分子植物（英文版）

基　　金：supported by the National Research Foundation Competitive Research Programme(NRF-CRP22-2019-0001);the intramural funding from Temasek Life Sciences Laboratory。

摘　　要：RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in eukaryotes.Many RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA metabolism.Increasing evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress adaptation.Here,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA metabolism.We also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.

关 键 词：RNA-binding proteins phase separation biomolecular condensates RNA metabolism development stress resilience 

分 类 号：Q94[生物学—植物学]