The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato  

作　　者：Shaofang Wu Chaoyi Hu Changan Zhu Yanfen Fan Jie Zhou Xiaojia Xia Kai Shi Yanhong Zhou Christine H.Foyer Jingquan Yu 

机构地区：[1]Department of Horticulture,Zijingang Campus,Zhejiang University,Hangzhou 310058,China [2]Hainan Institute,Zhejiang University,Yazhou Bay Science and Technology City,Sanya 572025,China [3]College of Horticulture,Northwest Agriculture&Forestry University,Xianyang 712100,China [4]School of Biosciences,College of Life and Environmental Sciences,University of Birmingham,Edgbaston B152TT,UK [5]Key Laboratory of Horticultural Plants Growth and Development,Agricultural Ministry of China,Zhejiang University,Hangzhou 310058,China

出　　处：《Molecular Plant》2024年第4期598-613,共16页分子植物（英文版）

基　　金：funded by the National Natural Science Foundation of China Projects of International Cooperation and Exchange(32020103013);the National Natural Science Foundation of China(32302532);the Modern Agro-industry Technology Research System of China(CARS-25-02A).

摘　　要：Jasmonates(JAs),a class of lipid-derived stress hormones,play a crucial role across an array of plant physiological processes and stress responses.Although JA signaling is thought to rely predominantly on the degradation of specific JAZ proteins by SCF^(COI1),it remains unclear whether other pathways are involved in the regulation of JAZ protein stability.Here,we report that PUB22,a plant U-box type E3 ubiquitin ligase,plays a critical role in the regulation of plant resistance against Helicoverpa armigera and other JA responses in tomato.Whereas COI1 physically interacts with JAZ1/2/5/7,PUB22 physically interacts with JAZ1/3/4/6.PUB22 ubiquitinates JAZ4 to promote its degradation via the 26S proteasome pathway.Importantly,we observed that pub22 mutants showreduced resistance to H.armigera,whereas jaz4 single mutants and jaz1 jaz3 jaz4 jaz6 quadruple mutants have enhanced resistance.The hypersensitivity of pub22 mutants to herbivores could be partially rescued by JAZ4 mutation.Moreover,we found that expression of PUB22 can be transcriptionally activated by MYC2,thus forming a positive feedback circuit in JA signaling.We noticed that the PUB22-JAZ4 module also regulates other JA responses,including defense against B.cinerea,inhibition of root elongation,and anthocyanin accumulation.Taken together,these results indicate that PUB22 plays a crucial role in plant growth and defense responses,together with COI1-regulated JA signaling,by targeting specific JAZs.

关 键 词：PUB22 JAZ COI1 MYC2 JA signaling Solanum lycopersicum 

分 类 号：Q94[生物学—植物学]