A viral movement protein targets host catalases for 26S proteasome-mediated degradation to facilitate viral infection and aphid transmission in wheat  

作　　者：Shuyuan Tian Qingting Song Wenmei Zhou Jingke Wang Yanbin Wang Wei An Yunfeng Wu Lei Zhao 

机构地区：[1]State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China [2]Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau,Ministry of Agriculture and Rural Affairs,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China [3]Key Laboratory of Plant Protection Resources and Pest Management,Ministry of Education,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China

出　　处：《Molecular Plant》2024年第4期614-630,共17页分子植物（英文版）

基　　金：supported by grants to Y.Wu and L.Z.from the Shaanxi Key Research and Development Program(No.2022KWZ-11);the Ministry of Science and Technology Plans to Introduce High-End Foreign Experts(G2022172015L);the National Natural Science Foundation of China(Nos.32372501 and 31701761).

摘　　要：The infection of host plants by many different viruses causes reactive oxygen species(Ros)accumulation and yellowing symptoms,but the mechanisms through which plant viruses counteract RoS-mediated immunity to facilitate infection and symptom development have not been fully elucidated.Most plant viruses are transmitted by insect vectors in the field,but the molecular mechanisms underlying virus-host-insect interactions are unclear.In this study,we investigated the interactions among wheat,barley yellow dwarf virus(BYDV),and its aphid vector and found that the BYDV movement protein(MP)interacts with both wheat catalases(CATs)and the 26S proteasomeubiquitin receptor non-ATPase regulatorysubunit2homolog(PSMD2)to facilitate the 26S proteasome-mediateddegradation of CATs,promotingviral infection,disease symptom development,and aphid transmission.Overexpression of the BYDV MP gene in wheat enhanced the degradation of CATs,which leading to increased accumulation of ROS and thereby enhanced viral infection.Interestingly,transgenic wheat lines overexpressing BYDV MP showed significantly reduced proliferation of wingless aphids and an increased number of winged aphids.Consistent with this observation,silencing of CAT genes also enhanced viral accumulation and reduced the proliferation of wingless aphids but increased the occurrence of winged aphids.In contrast,transgenic wheat plants overexpressing TaCAT1 exhibited the opposite changes and showed increases in grain size and weight upon infection with BYDV.Biochemical assays demonstrated that BYDV MP interacts with PSMD2 and promotes 26S proteasome-mediated degradation of TaCAT1 likely in a ubiquitination-independent manner.Collectively,our study reveals a molecular mechanism by which a plant virus manipulates the Ros production system of host plants to facilitate viral infection and transmission,shedding new light on the sophisticated interactions among viruses,host plants,and insect vectors.

关 键 词：babarley yellow dwarf virus movement protein reactive oxygen species APHID CATALASE 26S proteasomeubiquitin receptor PSMD2 

分 类 号：S512.1[农业科学—作物学]