鸡球虫重组蛋白rEnApiAP2对鸡免疫保护效果的观察  

Immunoprotective Efficacy of Recombinant Protein rEnApiAP2 against Coccidia in Chicken

作　　者：王乐乐[1,2] 王礼跃 蔡为民 康喜龙 冯茜茜 张知之[1,2] 范雪莲 朱玉 刘丹丹 许金俊 潘志明[2,3,4] 陶建平 WANG Lele;WANG Liyue;CAI Weimin;KANG Xilong;FENG Qianqian;ZHANG Zhizhi;FAN Xuelian;ZHU Yu;LIU Dandan;XU Jinjun;PAN Zhiming;TAO Jianping(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou 225009,China;Jiangsu Key Laboratory of Zoonosis,Yangzhou 225009,China;Suqian University,Suqian 223800,China)

机构地区：[1]扬州大学兽医学院,扬州225009 [2]江苏动物重要疫病与人兽共患病防控协同创新中心,扬州225009 [3]江苏省人兽共患病学重点实验室,扬州225009 [4]宿迁学院,宿迁223800

出　　处：《畜牧兽医学报》2024年第4期1716-1727,共12页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31972698);江苏省重点研发计划(现代农业,BE2021354);江苏省高校优势学科建设三期工程项目(2017);高等学校学科创新引智计划资助(D18007)。

摘　　要：将毒害艾美耳球虫重组蛋白rEnApiAP2单独免疫(单免)或分别与柔嫩艾美耳球虫重组蛋白rEtGAM56或rEtGAM59联合免疫(联免)雏鸡,同时设rEtGAM56单免、rEtGAM59单免、未免疫攻虫和空白组为对照,分别攻毒害艾美耳球虫或柔嫩艾美耳球虫,以成活率、排出血便数、平均增重、相对增重率、病变记分、卵囊减少率、抗球虫指数(ACI)和血清抗体水平为指标,评价rEnApiAP2单免及与rEtGAM联免的免疫保护效果。结果显示:各试验组的成活率均为100%;与未免疫攻虫组比较,各免疫组鸡排血便堆数减少、平均增重增加。在rEnApiAP2单免组间,低剂量组的相对增重率(82.36%)、卵囊减少率(73.88%)和抗球虫指数(155.26)均为最高,平均病变记分最低(1.71)。攻毒害艾美耳球虫后,联免组的ACI值均大于相应的单免组,其中rEnApiAP2与rEtGAM56联免组的ACI值(169.83)最高。攻柔嫩艾美耳球虫后,rEnApiAP2单免组的ACI值(128.37)低于rEtGAM56、rEtGAM59单免组(130.12、151.88),rEnApiAP2与rEtGAM59联免组的ACI值(141.62)接近于rEtGAM59单免组,rEnApiAP2与rEtGAM56联免组的ACI值(115.46)小于rEtGAM56单免组。各免疫组血清抗体水平均显著高于空白对照组(P<0.05)。rEnApiAP2对毒害艾美耳球虫具有一定的免疫保护效果,与rEtGAM联免可提高对毒害艾美耳球虫的免疫保护力。本研究结果为鸡球虫病重组亚单位疫苗研制奠定了基础。The recombinant protein rEnApiAP2 of Eimeria necatrix alone and in combination with the recombinant proteins rEtGAM56 or rEtGAM59 of E.tenella were used to immunize chicks.The groups immunized with rEtGAM56 or rEtGAM59 alone,and the groups unimmunized and challenged(UC)or unimmunized and unchallenged were used as controls.The chicks were infected with E.necatrix or E.tenella,respectively.The survival rate,bloody stool,weight gain(WG),relative weight gain(RWG),lesion score(LC),oocyst reduction(OR),anticoccidial index(ACI)and serum antibody level(SAL)were used as indexes to evaluate the immune protective effect of rEnApiAP2 alone and in combination with rEtGAM56 or rEtGAM59,respectively.The results showed that the survival rate of all groups was 100%,the total number of bloody stools excreted by chicks in the immunized groups was less than that in the UC group,and the WG of the immunized groups were higher than that of UC group.Among the groups immunized with rEnApiAP2 alone,the RWG,LC and ACI in the group immunized with low dosage was the highest(82.36%,73.88%and 155.26 respectively),and the LC was the lowest(1.71).Among the groups infected with E.necatrix,the ACI in the co-immunization groups was higher than that in the groups immunized with rEnApiAP2 or rEtGAM alone,of which the ACI in the group co-immunized with rEnApiAP2 and rEtGAM56 was the highest(169.83).Among the groups infected with E.tenella,the ACI in the group immunized with rEnApiAP2(128.37)was lower than that of the group immunized with rEtGAM56(130.12)and the group immunized with rEtGAM59(151.88).The ACI in the group co-immunized with rEnApiAP2 and rEtGAM59(141.62)was close to that in the group immunized with rEtGAM59 alone,and the ACI in the group co-immunized with rEnApiAP2 and rEtGAM56(115.46)was lower than that in the group immunized with rEtGAM56 alone.The SAL of all immunized groups was significantly higher than that of the control group(P<0.05).In conclusion,rEnApiAP2 protein had good immunogenicity and was able to induce effective immune pro

关 键 词：毒害艾美耳球虫 柔嫩艾美耳球虫 rEnApiAP2 rEtGAM 保护效果 

分 类 号：S855.9[农业科学—临床兽医学]