三七总皂苷和马齿苋多糖合用的抗炎功效评价  

Anti-Inflammatory Effect of Panax Notoginseng Saponins Combined with Portulaca Oleracea Polysaccharide

作　　者：夏金凤 陈影 杨万松 张钰雯[1] 陈真[1] XIA Jinfeng;CHEN Ying;YANG Wansong;ZHANG Yuwen;CHEN Zhen(Incutation Center for Scientific and Technology Achievement,Kunming Medical University,Kuming 650500,Yunnan,China;School of Humanities and Management,Kunming Medical University,Kunming 650500,Yunnan,China;Yunnan Yuefangji Biotechnology Co.,Ltd.,Kuming 650501,Yunnan,China)

机构地区：[1]昆明医科大学科技成果孵化中心,云南昆明650500 [2]昆明医科大学人文与管理学院,云南昆明650500 [3]云南悦芳季生物资源开发有限公司,云南昆明650501

出　　处：《香料香精化妆品》2024年第2期89-93,共5页Flavour Fragrance Cosmetics

摘　　要：为了探讨不同质量浓度下三七总皂苷和马齿苋多糖的协同抗炎组方,将不同质量浓度的三七总皂苷(1、5、25、125μg/mL)和马齿苋多糖(2、10、50、250μg/mL)两两复配,以100μg/mL地塞米松为阳性对照,通过测定炎症因子TNF-α的抑制率评估16种复配组合物的抗炎功效,并采用协同分数(synergy score值)系统定量评价复配组合物中2种成分的相互作用。结果显示,当质量浓度为125μg/mL的三七总皂苷与50μg/mL或250μg/mL的马齿苋多糖复配时,均对TNF-α具有显著的抑制效应,抑制率分别为45.18%和61.42%,其抑制作用与100μg/mL的地塞米松(抑制率为53.17%)无显著性差异(P>0.05),synergy score值分别为15.58和26.44。综上,125μg/mL的三七总皂苷与50μg/mL或250μg/mL的马齿苋多糖合用时协同抑制TNF-α的产生,但其余14种更低剂量的复配组合仅表现为相加作用。To investigate the synergistic anti-inflammatory effects of Panax notoginseng saponins(PNS)and Portulaca oleracea polysaccharide(POP)at different dosage concentrations.PNS(1,5,25,125μg/mL)and POP(2,10,50,250μg/mL)were respectively combined in pairs at different concentrations,and 100μg/mL dexamethasone was used as a positive control.The inhibition rate of TNF-αof 16 combinations was studied,and the interaction of the two components was quantitatively evaluated by the synergy score system.PNS at 125μg/mL combined with POP at 50μg/mL or 250μg/mL effectively suppressed TNF-αproduction,and the inhibitory rates were 45.18%and 61.42%respectively.The inhibitory effect was not significantly different from that of dexamethasone 100μg/mL(53.17%)(P>0.05).Synergy scores were 15.58 and 26.44,respectively.It was suggested that 125μg/mL PNS combined with 50μg/mL or 250μg/mL POP could synergically inhibit the production of TNF-α,but the other 14 lower dose combinations only showed the additive effect.

关 键 词：协同作用 三七总皂苷 马齿苋多糖 炎症因子 

分 类 号：TQ658[化学工程—精细化工]