柠檬酸修饰玻碳电极检测卡比多巴含量研究  

Determination of Carbidopa Using Glassy Carbon Electrode Modified with Citric Acid

作　　者：张英 蔡婷婷 张晋原 邹宇 任旺 ZHANG Ying;CAI Tingting;ZHANG Jinyuan;ZOU Yu;REN Wang(School of Chemistry and Environmental Engineering,Sichuan University of Science&Engineering,Zigong 643000,China)

机构地区：[1]四川轻化工大学化学与环境工程学院,四川自贡643000

出　　处：《四川轻化工大学学报（自然科学版）》2024年第2期9-15,共7页Journal of Sichuan University of Science & Engineering(Natural Science Edition)

基　　金：国家自然科学基金项目(22064005);四川省自然科学基金面上项目(2022NSFSC0103);乡村低成本环境治理技术四川省高校重点实验室开放项目(XCH2022ZB-04);四川轻化工大学研究生创新基金项目(Y2022027);四川轻化工大学大学生创新基金项目(S20221062209)。

摘　　要：通过恒电位沉积法在玻碳电极表面分别修饰柠檬酸、对苯二胺、对氨基苯磺酸和L-谷氨酰胺,制备4种修饰电极用于卡比多巴(CD)的检测。实验结果表明,柠檬酸修饰电极对CD的检测显示了更好的选择性和较高的灵敏度。采用循环伏安和交流阻抗等对修饰电极的电子传导能力进行表征。运用差分脉冲伏安(DPV)研究了柠檬酸修饰电极在左旋多巴(LD)共存下对CD的检测性能。在pH为5.8的磷酸盐缓冲液中,CD在0.65 V处的氧化峰电流与其浓度在4.0×10^(-6)~1.0×10^(-4) mol/L范围内呈线性关系。利用柠檬酸修饰电极对复方卡左多巴片剂中的CD进行检测,相对误差在±5%范围内。Four kinds of glassy carbon electrode are prepared to determine carbidopa(CD),which are modified with citric acid,p-phenylenediamine,p-aminobenzene sulfonic acid or L-glutamine by constant potential deposition method.The experiment results show that the glassy carbon electrode modified with citric acid(CA/GCE)exhibits a better selectivity and high sensitivity compared with other electrodes,when it’s used to determine the CD.The electron transfer ability of the modified electrode is characterized by using the cyclic voltammetry and electrochemical impedance spectra.The detection ability of CA/GCE electrode for CD in CD-LD compound tablets is investigated by differential pulse voltammetry(DPV).The oxidation peak current at 0.65 V in the phosphate buffer solutions(pH=5.8)is linearly dependent on the CD concentrations in the range of 4.0×10^(-6)~1.0×10^(-4) mol/L.The proposed electrode shows potential applications in the detection of CD in CD-LD compound tablets,and the relative error is within±5%.

关 键 词：卡比多巴 电化学检测 修饰电极 柠檬酸 

分 类 号：O657.1[理学—分析化学]