基于毒理基因组学的工业废气颗粒物毒性分析方法及应用  

Toxicity Analysis Method and Application of Industrial Exhaust Particulate Matter Based on Toxicology Genomics

作　　者：何剑[1] 吴兴刚 王珊珊[1] 郑兴[1] 刘玉玲[1] HE Jian;WU Xinggang;WANG Shanshan;ZHENG Xing;LIU Yuling(Department of Municipal and Environmental Engineering,Xi′an University of Technology,Xi′an 710048,China)

机构地区：[1]西安理工大学市政与环境工程系,陕西西安710048

出　　处：《环境科学研究》2024年第4期719-727,共9页Research of Environmental Sciences

基　　金：国家自然科学基金项目(No.52100104);陕西省重点研发计划项目(No.2019ZDLSF05-05-01-01)。

摘　　要：为了解决现有颗粒物毒性检测方法存在的成本高、周期长、过程复杂等问题,引入毒理基因组学分析方法,提出工业废气颗粒物样品预处理方法,基于酶标仪开展毒性测试,利用基因热图分析、应激模式反应和毒性终点深入探究颗粒物的毒性作用机制,验证了方法的可行性,并将该方法应用于某烧结机头电除尘器内4个电场(1号、2号、3号、4号)中颗粒物的毒性变化规律分析.结果表明:①对于有机组分,其主要诱导大肠杆菌细胞发生氧化应激反应和潜在的蛋白应激反应,颗粒物有机组分毒性呈4号电场>3号电场>1号电场>2号电场的特征,4号电场的颗粒物毒性终点最小,为3.01×10^(−10) mg/mL.②对于无机组分,4个电场(1号、2号、3号、4号)主要诱导大肠杆菌细胞分别发生普遍应激、DNA应激、氧化应激和蛋白应激,颗粒物无机组分毒性大小呈4号电场>2号电场>1号电场>3号电场的特征,4号电场的颗粒物毒性终点最小,为8.44×10^(−6) mg/mL.研究显示,基于毒理基因组学的工业废气颗粒物毒性分析方法不仅具有准确性,还能更加全面地阐述潜在的毒性模式,实现了工业废气颗粒物毒性的快捷可靠分析,可为后续相关研究提供理论支撑.To avoid high cost,long cycles and complex processes in existing particle toxicity detection methods,toxicological genomics analysis was introduced,and an industrial waste gas particulate matter samples pretreatment method proposed.A toxicity test was conducted based on the microplate reader where the toxic action mechanism of particulate matter was further explored by gene heat map analysis,and the stress mode reaction and toxicity endpoint determined.The feasibility of the method was verified and the method was applied to analysis of particle toxic variation in the electrostatic precipitator of a sintering machine head four electric fields(No.1,No.2,No.3,No.4).We showed that:(1)For organic components,it mainly induces oxidative stress and potential protein stress in Escherichia coli cells.Order of organic components of particulate matter toxicity was No.4 electric field>No.3 electric field>No.1 electric field>No.2 electric field.The No.4 electric field minimum particle toxicity endpoint was 3.01×10^(−10 )mg/mL.(2)For inorganic components,four electric fields(No.1,No.2,No.3,No.4)mainly induced general stress,DNA stress,oxidative stress and protein stress in E.coli cells.Order of inorganic components particulate matter toxicity was No.4 electric field>No.2 electric field>No.1 electric field>No.3 electric field.The No.4 electric field minimum particle toxicity endpoint was 8.44×10^(−6) mg/mL.The research shows that the analysis method for industrial waste gas particle toxicity based on toxicology genomics is not only accurate,but also provides a more comprehensive explanation of the potential toxicity model.This method enables rapid and reliable analysis of industrial waste gas particle toxicity and provides theoretical support for subsequent related research.

关 键 词：工业废气颗粒物 毒理基因组学 毒性分析 毒性机制 

分 类 号：X513[环境科学与工程—环境工程]