基于制片竹秆软化方法研究  

Anatomical Research Based on Methods of Bamboo Culm Softening

作　　者：肖姣 胡思思 孔令辉 胡添翼 张文元[1,2] 于芬 XIAO Jiao;HU Si-si;KONG Ling-hui;HU Tian-yi;ZHANG Wen-yuan;YU Fen(College of Forestry,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization,Nanchang 330045,Jiangxi,China)

机构地区：[1]江西农业大学林学院,江西南昌330045 [2]江西省竹子种质资源与利用重点实验室,江西南昌330045

出　　处：《竹子学报》2024年第1期36-43,共8页Journal of Bamboo Research

基　　金：国家自然科学基金项目(31960336,31460177)。

摘　　要：【目的】为了探讨不同软化方式对竹材结构和主要组分的影响,筛选适合不同研究目的的最佳软化方法。【方法】以厚竹(Phyllostachys edulis‘Pachyloen’)竹秆为研究对象,采用常规石蜡制片结合显微技术和紫外光谱分析技术进行研究。【结果】除了三甘醇和氢氟酸软化的材料容易破裂外,水以及其他化学试剂软化均可获得较为完整、清晰的竹秆结构。且乙二胺为竹秆显微切片制备的最适软化介质。【结论】10%乙二胺100℃水浴软化最为方便快捷,适于以结构研究为目的,木质化程度高的竹类植物显微制片。对于竹秆组成成分研究,纯水100℃水浴软化为最佳选择。该文为竹材利用基础研究提供了参考依据。【Objective】In order to explore the effects of different softening methods on the structure and main components of bamboo timber,and select the best softening method suitable for different research purposes.【Method】Microsections of bamboo culm of Phyllostachys edulis‘Pachyloen’were taken as the research object,and the paraffin section method with microscopy techniques and UV spectroscopy was used.【Result】The results showed that the structure was complete and clear after softened by water and chemical softener except triethylene glycol and hydrofluoric acid,which led bamboo culm structure rupture.Besides,ethylenediamine was the optimal softener for the preparation of microsection of bamboo culm.【Conclusion】10%ethylenediamine softening in a water bath at 100℃was the most convenient and fastest,which was suitable for microscopic sectioning of bamboo plants with high degree of lignification for structural research purposes.For component investigation of bamboo culm,the optimal softening treatment was being softened in a 100℃distilled water bath.The results of this study provides a reference basis for basic research of bamboo culm utilization.

关 键 词：竹材 软化 切片 结构 组分 

分 类 号：S781.9[农业科学—木材科学与技术]