Generation of mitochondrial replacement monkeys by female pronucleus transfer  

作　　者：Chun-Yang Li Xing-Chen Liu Yu-Zhuo Li Yan Wang Yan-Hong Nie Yu-Ting Xu Xiao-Tong Zhang Yong Lu Qiang Sun 

机构地区：[1]Institute of Neuroscience,Center for Excellence in Brain Science and Intelligence Technology,State Key Laboratory of Neuroscience,Chinese Academy of Sciences,Shanghai 200031,China [2]Key Laboratory of Genetic Evolution and Animal Models,Chinese Academy of Sciences,Kunming 650201,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Zoological Research》2024年第2期292-298,共7页动物学研究（英文）

基　　金：supported by the National Natural Science Foundation of China (82021001,31825018);National Key Research and Development Program of China (2022YFF0710901);Shanghai Municipal Science and Technology Major Project (2018SHZDZX05);Strategic Priority Research Program of the Chinese Academy of Sciences (XDB32060100);Biological Resources Program of Chinese Academy of Sciences (KFJ-BRP-005);National Science and Technology Innovation 2030 Major Program 2021ZD0200900。

摘　　要：Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA diseases.While pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously reported.In this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear transfer.These individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during development.The successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.

关 键 词：Non-human primates Mitochondrial replacement Female pronuclear transfer 

分 类 号：R596[医药卫生—内科学] R-332[医药卫生—临床医学]