碳纳米颗粒增敏的分子印迹电化学传感器检测阿奇霉素的研究  

Detection of azithromycin by molecularly imprinted electrochemical sensitization with carbon nanoparticles

作　　者：冯旭 郭航雨 覃丹凤 李利军[1,2] FENG Xu;GUO Hangyu;QIN Danfeng;LI Lijun(School of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Guangxi Key Laboratory of Green Processing of Sugar Resources(Guangxi University of Science and Technology),Liuzhou 545006,China;Medical Faculty,Guangxi University of Science and Technology,Liuzhou 545006,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西糖资源绿色加工重点实验室(广西科技大学),广西柳州545006 [3]广西科技大学医学部,广西柳州545005

出　　处：《广西科技大学学报》2024年第2期87-96,共10页Journal of Guangxi University of Science and Technology

基　　金：国家自然科学基金项目(82260701);广西自然科学基金重点项目(2019GXNSFDA245025)资助。

摘　　要：以磺化沥青(sulfonsted bitumen,SP)作为碳源,通过纯化和高温碳化法制备碳纳米颗粒(carbon nanoparticles,CNPs),将CNPs分散在壳聚糖(chitosan,CS)溶液中,并用滴涂法修饰在金电极(Au)上来得到CNPs-CS/Au。以阿奇霉素(azithromycin,AZM)为模板分子、多巴胺(dopamine,DA)为功能单体,利用电聚合法在CNPs-CS/Au电极上制备了AZM分子印迹薄膜,构建了高灵敏度且专一性识别AZM分子的电化学传感器(MIP/CNPs-CS/Au)。分别采用扫描电镜(scanning electron microscope,SEM)、X-射线衍射(X-ray diffraction,XRD)、X-射线光电子能谱(Xray photoelectron energy spectroscopy,XPS)、循环伏安法(cyclic voltammetry,CV)、电化学阻抗法(electrochemical impedance method,EIS)和差示脉冲阳极溶出伏安法(differential pulse anode dissolution voltammotry,DPASV)对MIP/CNPs-CS/Au进行表征。结果表明,该传感器的电流值(I_(pa))与AZM浓度(C)分别在0.005~<5.000μmol/L和5.000~30.000μmol/L范围内呈现良好的线性关系,检测限(limit of detection,LOD)较低(3.89 nmol/L,S/N=3)。该传感器可用于实际样品中AZM的检测,加标回收率为97.10%~103.96%,相对标准偏差(relative standard deviation,RSD)为1.10%~2.07%。Carbon nanoparticles(CNPs)were prepared by purification and high-temperature carbonization using sulfonated asphalt(SP)as the carbon source.CNPs were dispersed in chitosan(CS)solution and modified on a gold electrode(Au)using a drop coating method to obtain CNPs-CS/Au.Using azithromycin(AZM)as a template molecule and dopamine(DA)as a functional monomer,AZM molecularly imprinted thin films were prepared on CNPs-CS/Au electrodes using electro polymerization method.A highly sensitive and specific electrochemical sensor(MIP/CNPs-CS/Au)for recognizing AZM molecules was constructed.MIP/CNPs-CS/Au was characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD),X-ray photoelectron energy spectroscopy(XPS),cyclic voltammetry(CV),electrochemical impedance spectroscopy(EIS),and differential pulse anode stripping voltammetry(DPASV),respectively.The results show that the current value(I_(pa))and AZM concentration(C)of the sensor show a good linear relationship in the range of 0.005~<5.000μmol/L and 5.000~30.000μmol/L,respectively.The limit of detection(LOD)is low(3.89 nmol/L,S/N=3).This sensor can be used for the detection of AZM in actual samples,with the spiked recovery rate of 97.10%~103.96%and a relative standard deviation(RSD)of 1.10%~2.07%.

关 键 词：碳纳米颗粒 分子印迹聚合物 电化学传感器 阿奇霉素 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]