IVF-ET胚胎培养液中sHLA-G、MMP-9与胚胎质量及临床妊娠关系  

Correlation between the levels of the soluble human leukocyte antigen G and the matrix metalloproteinase in the embryo culture medium of women after in vitro fertilization embryo transfer and their embryo quality and clinical pregnancy

作　　者：张楠 刘聪 李伟伟 史鸿志 ZHANG Nan;LIU Cong;LI Weiwei;SHI Hongzhi(Maternal and Child Health Care Hospital of Qinhuangdao,Hebei Province,066000)

机构地区：[1]河北省秦皇岛市妇幼保健院,066000

出　　处：《中国计划生育学杂志》2024年第4期834-838,共5页Chinese Journal of Family Planning

基　　金：秦皇岛市卫生健康委员会社会发展与乡村科技科研项目(202301A046)。

摘　　要：目的:分析体外受精-胚胎移植(IVF-ET)胚胎培养液中可溶性人类白细胞抗原G(sHLA-G)、基质金属蛋白酶(MMP-9)与胚胎质量、临床妊娠的关系。方法:收集2017年1月-2023年1月在本院生殖中心接受IVF-ET治疗的女性患者107例临床资料及标本,新鲜胚胎移植后收集胚胎培养液,分别取未移植的正常受精胚胎中优质胚胎、中等胚胎、劣质胚胎对应胚胎培养液,酶联免疫吸附实验检测胚胎培养液中sHLA-G、MMP-9水平,比较不同胚胎质量对应的胚胎培养液sHLA-G、MMP-9水平及其相关性;记录IVF-ET患者临床妊娠情况并分为临床妊娠组、未妊娠组,比较两组胚胎培养液中的sHLA-G、MMP-9水平及其他临床资料,logistic回归分析胚胎培养液中sHLA-G、MMP-9水平与临床妊娠关系。结果:107例未移植的正常受精胚胎中共收集127份胚胎培养液标本,其中优质胚胎培养液44份、中等胚胎培养液52份、劣质胚胎培养液30份,其胚胎培养液sHLA-G、MMP-9水平逐渐降低,胚胎培养液sHLA-G、MMP-9水平与胚胎质量正相关(均P<0.01)。107例IVF-ET获得临床妊娠69例,临床妊娠率64.6%,未妊娠组胚胎培养液sHLA-G(8.11±1.63 ng/ml)、MMP-9水平(0.45±0.17 ng/ml)均低于临床妊娠组(10.63±2.41 ng/ml、0.61±0.12 ng/ml),第三日卵裂球数(6.0±1.8个)、优质胚胎率(45.6±12.1)%低于临床妊娠组(8.9±1.8个、64.8%±15.9%)(均P<0.05);logistic回归分析,sHLA-G、MMP-9、第三日卵裂球数、优质胚胎率等均是影响IVF-ET患者临床妊娠因素。结论:IVF-ET患者胚胎培养液中sHLA-G、MMP-9水平与胚胎质量正相关,sHLA-G、MMP-9、第三日卵裂球数、优质胚胎率等降低均是IVF-ET患者临床妊娠影响因素。Objective:To analyze the correlation between the levels of the soluble human leukocyte antigen G(sHLAG)and the matrix metalloproteinase(MMP-9)in the embryo culture medium of women after in vitro fertilization embryo transfer(IVF-ET)and their embryo quality and clinical pregnancy.Methods:The clinical data,the samples,and the embryo culture medium after fresh embryo transfer of 107 women who had received IVF-ET treatment in the reproductive center from January 2017 to January 2023 were collected.The embryo culture mediums were collected from the high-quality embryos,medium embryos and inferior embryos in the normal fertilized embryos without transplantation.The enzyme-linked immunosorbent assay was used to detect the levels of sHLA-Gand MMP-9in the embryo culture medium.The levels of the sHLA-G and MMP-9 in the embryo culture medium were compared among the women with different embryo quality.The correlation between the levels of the sHLA-G and MMP-9in the embryo culture medium and their embryo quality was analyzed.The clinical pregnancy situations of the women with IVF-ET were recorded,and the women were divided into group A(women with clinical pregnancy)and group B(women without clinical pregnancy).The levels of sHLA-Gand MMP-9 in the embryo culture medium and the other clinical data of the women were compared between the two groups.Logistic regression analysis was used to analyze the correlation between the levels of sHLA-G and MMP-9 in the embryo culture medium of the women and their clinical pregnancy.Results:A total of 127 samples of the embryo culture medium were collected from 107 normal fertilized embryos without transfer,including 44 embryo culture mediums of the high-quality embryo,52 embryo culture mediums of the medium-quality embryo and 30 embryo culture mediums of the poor-quality embryo.The levels of sHLA-G and MMP-9in the embryo culture medium of the high-quality embryo,the medium-quality embryo and the poor-quality embryo had decreased gradually.The levels of sHLA-G and MMP-9in the embryo culture m

关 键 词：体外受精-胚胎移植 胚胎培养液 可溶性人类白细胞抗原G 基质金属蛋白酶 胚胎质量 临床妊娠 相关性 

分 类 号：R714.8[医药卫生—妇产科学]