稽留流产患者血清、绒毛与蜕膜组织中SLIT2的表达及临床意义  

The expression and the clinical significance of neurodirecting factor 2 in serum,villi tissues and decidual tissues of women with missed abortion

作　　者：谢瑞萍 杨彩霞 范俊梅 李新燕 XIE Ruiping;YANG Caixia;FAN Junmei;LI Xinyan(Shanxi Provincial Children's Hospital(Shanxi Maternal and Child Health Care Hospital),Taiyuan,Shanxi Province,030001)

机构地区：[1]山西省儿童医院,山西省妇幼保健院,太原030001

出　　处：《中国计划生育学杂志》2024年第4期856-860,共5页Chinese Journal of Family Planning

摘　　要：目的:探讨稽留流产患者血清、绒毛与蜕膜组织中神经导向因子2(SLIT2)表达水平及临床意义。方法:收集2020年10月-2023年10月本院收治的196例稽留流产患者临床资料为稽留流产组,正常早孕终止妊娠者120例为对照组。酶联免疫吸附法检测血清SLIT2水平,实时荧光定量聚合酶链式反应检测绒毛组织、蜕膜组织中SLIT2 mRNA表达水平;采用Pearson相关分析探讨稽留流产不同标本中SLIT2表达与血清hCG、VEGF、IL-6水平的相关性,受试者工作特性(ROC)曲线评估不同标本中SLIT2对稽留流产的诊断价值。结果:稽留流产组血清SLIT2水平(6.04±1.12ng/ml)、绒毛(0.62±0.16)及蜕膜组织(0.45±0.14)SLIT2 mRNA表达均低于对照组(9.15±1.86ng/ml、1.86±0.33、1.54±0.27),血清绒毛膜促性腺激素(hCG)(41.16±5.23MIU/mD)、血管内皮生长因子(VEGF)(91.05±9.26pg/ml)水平低于对照组(86.54±8.39MIU/mD、124.26±12.73pg/ml),白细胞介素-6(IL-6)(47.51±5.08pg/ml)水平高于对照组(10.37±1.44pg/ml);血清、绒毛组织、蜕膜组织中SLIT2水平与血清hCG、VEGF水平均呈正相关,与IL-6水平呈负相关(均P<0.05)。血清、绒毛组织、蜕膜组织中SLIT2诊断稽留流产的曲线下面积(AUC)分别为0.771、0.903、0.865(0.814~0.916),截点值分别为7.59ng/ml、0.99、1.24,特异度分别为57.0%、87.0%、66.9%,灵敏度分别为92.6%、84.7%、92.6%。结论:稽留流产患者血清、绒毛组织、蜕膜组织中SLIT2水平均降低,且与机体炎症反应、胎盘血管生成有关,有望作为诊断稽留流产的潜在标记物。Objective:To explore the expression and the clinical significance of the neurodirecting factor 2(SLIT2)in serum,villi tissues and decidual tissues of women with missed abortion.Methods:The clinical data of 196 women with missed abortion(in study group)admitted to hospital from October 2020 to October 2023were collected,and 120 women with normal early pregnancy termination were selected in control group during the same period.The serum SLIT2 level of the women in the two groups were detected by ELISA.Real-time fluorescent quantitative polymerase chain reaction was used to detect the expression level of SLIT2 mRNA in villi tissues and decidua tissues of the women in the two groups.Pearson correlation analysis was used to investigate the correlation between theSLIT2 expression level in serum,villi tissues and decidual tissues of the women with missed abortion and their levels of the serum human chorionic gonadotropin(hCG),vascular endothelial growth factor(VEGF)and interleukin-6(IL-6).Receiver operator characteristic(ROC)curve was used to evaluate the diagnostic value of the SLIT2 level in serum,villi tissues and decidual tissues of the pregnancy women for their missed abortion.Results:The SLIT2 level in serum(6.04±1.12ng/ml),in villi tissues(0.62±0.16ng/ml)and in decidual tissues(0.45±0.14ng/ml)of the women in the study group were significantly lower than those(9.15±1.86ng/ml,1.86±0.33ng/ml and 1.54±0.27ng/ml)of the women in the control group.The SLIT2 mRNA expressions of the women in serum,in villi tissues and in decidual tissues of the women in the study group were significantly lower than those of the women in the control group.The levels of hCG(41.16±5.23MIU/mD)and VEGF(91.05±9.26pg/ml)of the women in the study group were significantly lower than those(86.54±8.39MIU/mD and 124.26±12.73pg/ml)of the women in the control group.The level of IL-6(47.51±5.08pg/ml)of the women in the study group was significantly higher than that(10.37±1.44pg/ml) of the women in the control group.The SLIT2 levels of the wo

关 键 词：稽留流产 神经导向因子2 血清 绒毛组织 蜕膜组织 诊断 

分 类 号：R714.21[医药卫生—妇产科学]